流式细胞仪分析比较基因敲入和转基因小鼠前列腺癌模型  

Flow cytometry analysis of transgenic and knock-in prostate cancer mouse models

作　　者：武国军[1,2] 李晓武[1] 朱李兵[1] 袁建林[1] 王禾[1] 秦荣良[1] 于磊[1] 秦卫军[1] 张运涛[1] 

机构地区：[1]第四军医大学西京医院泌尿外科,陕西西安710032 [2]加拿大西安大略大学维多利亚医院外科

出　　处：《中国现代医学杂志》2009年第10期1499-1501,共3页China Journal of Modern Medicine

基　　金：军队医药卫生"十一五"科研项目(No:06H040);第四军医大学优秀博士学位论文课题资助项目

摘　　要：目的利用流式细胞仪技术分析比较基因敲入(KIMAP)和转基因(TGMAP)小鼠前列腺癌模型。方法对来源于KIMAP和TGMAP的肿瘤标本进行了流式细胞仪分析,并与临床前列腺癌进行了比较。结果来源于TGMAP(n=8例)和KIMAP(n=9例)的小鼠前列腺癌标本流式细胞仪分析显示,多数KIMAP肿瘤为双倍体,与临床相近,而所有TGMAP肿瘤均为非整倍体。结论由于KIMAP模型成功地模仿了人类前列腺癌特征,在前列腺癌临床前期研究中具有潜在的应用价值。[Objective] This study used flow cytometry DNA analysis to measure and compare the new knock-in mouse adenocarcinoma prostate model （KIMAP） with transgenic mouse adenocareinoma prostate model （TGMAP） and human prostate cancer （CAP）. [Methods] Flow cytometry DNA analysis was performed on malignant prostate tissues obtained from both TGMAP and KIMAP models and compared with human CaP. [Results]Mice with CaP from TGMAP （n =8） and KIMAP （n = 9） models showed different results. Flow cytometry analysis demonstrated that the majority of tumor tissues in KIMAP were diploid, analogous to the human condition, while all the TGMAP mice showed aneuploid tumors. [ Conclusion] Due to the effective mimicry of human CaP tumors demonstrated by the PSP-KIMAP model, this study advocates its use in the clinical study of human CaP.

关 键 词：前列腺 肿瘤 小鼠模型 

分 类 号：R737.25[医药卫生—肿瘤] Q756[医药卫生—临床医学]