壳聚糖-海藻酸钠微胶囊固定化木聚糖酶的研究  

Enzymatic Characters of Free Xylanase and Immobilized Xylanase,Research on the Immobilized Xylanase with Chitosan-sodium Alginate Microcapsule

作　　者：王娜[1] 朱启忠[1] 任延刚[1] 张瑞景[1] 高原[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209

出　　处：《安徽农业科学》2009年第16期7318-7319,7325,共3页Journal of Anhui Agricultural Sciences

基　　金：山东大学威海分校科研立项项目(A08001)

摘　　要：[目的]研究游离酶和壳聚糖-海藻酸钠微胶囊固定化酶的酶学性质。[方法]以壳聚糖和海藻酸钠为载体,利用微胶囊技术固定木聚糖酶。[结果]结果表明,壳聚糖-海藻酸钠微胶囊法固定化木聚糖酶的固定率为39.4%,固定化酶和游离酶的Km值分别为0.765和0.687 g/L,固定化酶的热稳定性、重复操作稳定性和贮藏稳定性有了明显提高。[结论]采用壳聚糖-海藻酸钠微胶囊法固定化木聚糖酶具有一定的应用价值。[ Objective ] The enzymatic property of free Xylanaso and immobilized xylanase was researched. [ Method ] The sodium alginate and ehitosan being taken as carrier, the microcapsu]e technique was applied to immobilize xylanase. [ Results ] The results indicated that the recovery percentage of enzyme activity was 39.4% when the xylanase was immobilized with the sodium alginate-chitosan microcapsule, and the Km value of immobilized xylanaso and free xylanase were 0.765 and 0.687 g/L, respectively. And also, temperature stability, operation stability and storage stability of immobilized xylanase were obviously improved. [ Conclusion ] It was concluded that the method of the sodium alginate-chitosan microcapsule immobilizing xylanase had the value in industry.

关 键 词：海藻酸钠 壳聚糖 固定化 木聚糖酶 

分 类 号：Q814[生物学—生物工程]