erbB-2表达抑制与表皮生长因子刺激对信号转导与转录激活分子及细胞周期蛋白D1的影响  

EFFECTS OF erbB-2 REPRESSION AND EGF STIMULATION ON THE LEVEL OF STAT1 AND CYCLIN D1 PROTEIN

作　　者：姜琨[1,2] 秦樾 童坦君[1,2] 

机构地区：[1]北京医科大学生物化学与分子生物学系 [2]美国耶鲁大学医学院病理学系

出　　处：《北京医科大学学报》1998年第3期205-206,286,共3页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：目的：观察胃癌细胞内原癌基因ｅｒｂＢ２表达降低前后信号转导与转录激活分子（ＳＴＡＴ１）和细胞周期蛋白Ｄ１（ｃｙｃｌｉｎＤ１）对表皮生长因子（ＥＧＦ）刺激的反应性。方法：采用Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ检测ｅｒｂＢ２表达下调前后及ＥＧＦ刺激前后ＳＴＡＴ１和ｃｙｃｌｉｎＤ１水平。结果：ｅｒｂＢ２表达抑制对ＳＴＡＴ１的ＥＧＦ反应性无显著影响；ｅｒｂＢ２表达下调后ｃｙｃｌｉｎＤ１的ＥＧＦ可诱导性显著降低。结论：ｅｒｂＢ２表达降低可抑制ＥＧＦ对ｃｙｃｌｉｎＤ１的诱导效应。Objective: To investigate the protein level of STAT1 and cyclin D1 following EGF stimulation before and after the repression of erbB-2 gene. Methods: Western blotting analysis of STAT1 and cyclin D1 following EGF stimulation before and after the decrease on erbB-2 expression. Results: Alteration on erbB-2 expression has little effect on the level of STAT1 upon EGF stimulation; induction of cyclin D1 upon EGF stimulation significantly decreased after the repression of erbB2. Conclusion: Decrease of erbB2 expression can suppress the EGFinduction of cyclin D1.

关 键 词：胃肿瘤 原癌基因 表皮生长因子 细胞周期蛋白 

分 类 号：R735.2[医药卫生—肿瘤]