粘附分子P选择素单克隆抗体抑制胃癌转移的实验研究  

Inhibition of human stomach cancer metastasis in vivo by anti P selectin monoclonal antibody

作　　者：陈金联[1,2,3] 章月星[1,2,3] 储榆德 周同[1,2,3] 许蕙敏 程俊[1,2,3] 李明烈 

机构地区：[1]上海市第六人民医院 [2]上海市肿瘤研究所 [3]上海第二医科大学附属瑞金医院

出　　处：《中华医学杂志》1998年第6期437-439,共3页National Medical Journal of China

摘　　要：目的研究Ｐ选择素单克隆抗体对ＳＣＩＤ小鼠胃癌的抗转移作用。方法用人胃癌组织块ＳＧＣ７９０１原位移植ＳＣＩＤ小鼠制作转移模型。术后第３天开始，动物分别静脉注射Ｐ选择素单克隆抗体（单抗组，９只鼠），每次１００μｇ，每周２次，共３周；或ＰＢＳ液体（ＰＢＳ组，１１只鼠），每次１００μｌ，每周２次，共３周。术后第６周末，所有动物处死，取肿大淋巴结和可疑转移脏器作病理学检查。结果ＰＢＳ组肿瘤转移（淋巴结、肝、肺等脏器）率为９０９％（１０／１１），单抗组转移率为２２２％（２／９）。逆转录聚合酶链反应（ＲＴＰＣＲ）测定发现，ＳＣＩＤ小鼠有癌转移者其胃癌组织中Ｐ选择素ｍＲＮＡ基因表达较不伴癌转移者明显增强，正常对照鼠胃组织Ｐ选择素ｍＲＮＡ表达水平较低。结论Ｐ选择素及其基因ｍＲＮＡ表达与肿瘤转移相关，Ｐ选择素单克隆抗体具有抗肿瘤转移作用。Objective To study the role of cell adhesion molecule P selectin monoclonal antibody (Mab)in tumor metastasis of an orthotopic implantation metastatic model.Methods SCID mice were implanted orthotopically using SGC 7901 human stomach carcinoma intact tissue.Three days later,animals were given i.v.injections of PBS or P selectin Mab (100μg/injection) twice weekly for 3 weeks.42 days after operation, all animals were sacrificed.Tissues from stomach and other organs were obtained for histopathological evaluation.Results Ten of the animals ( n =11) treated with PBS developed metastatic tumors in the regional lymph nodes,liver,and lung. In contrast,2 of the animals ( n =9)treated with P selectin Mab developed metastatic tumors in the organs examined. The level of P、selectin mRNA expression determined by RT PCR in gastric carcinoma tissue of SCID mice with tumor metastas is was higher than that without metastasis. Conclusions The expression of P selectin adhesion moleculeis associated with tumor metastasis,and the metastasis can be arrested by the Mab.

关 键 词：膜糖蛋白类 肿瘤转移 胃肿瘤 粘附分子 P选择素 

分 类 号：R735.205[医药卫生—肿瘤]