眼外肌局部注射IGF-Ⅰ的组织学变化  

Histology of extraocular muscle after direct injection of insulin-like growth factor-Ⅰ

作　　者：许金玲[1] 余新平[1] 陈洁[1] 张芳[1] 余焕云[1] 高军[1] 

机构地区：[1]温州医学院附属眼视光医院,325027

出　　处：《眼科研究》2009年第6期477-480,共4页Chinese Ophthalmic Research

基　　金：温州医学院眼视光学院科研启动经费(KYQD070402);浙江省教育厅科研项目(20070995)资助

摘　　要：目的探讨局部注射胰岛素样生长因子Ⅰ(IGF-Ⅰ)后眼外肌组织学变化,评估局部用药对眼部组织的安全性。方法7只成年新西兰大白兔,双眼上直肌随机注射0.05mL(10μg)IGF-Ⅰ或0.05mL生理盐水,1周后获取上直肌行组织病理学检查。结果实验组和对照组眼球巩膜及色素膜未见明显炎性细胞浸润;实验组眶层纤维周径(120.24±30.76)μm与对照组(108.48±25.64)μm比较差异有统计学意义(t=6.734,P=0.000)。2组球层眼外肌肌纤维周径差异无统计学意义(t=1.527,P=0.127)。实验组的球层和眶层纤维大细胞和小细胞的比例与对照组差异有统计学意义。实验组球层和眶层眼外肌活化卫星细胞的比例较对照组均显著提高;2组眼外肌球层肌纤维的活化卫星细胞比例较眶层显著提高。结论局部注射10μgIGF-Ⅰ对眼部组织无明显不良反应,局部应用对眼外肌和眼球是安全的。IGF-Ⅰ能使眼外肌肌纤维快速增生,激活肌卫星细胞。球层和眶层眼外肌肌纤维对IGF-Ⅰ的反应可能有所不同。Objective Insulin-like growth factor （IGF） is verified to promote the proliferation of skeletal muscle and repairing of injury of skeletal muscle. Research showed that the contraction ability of extraocular muscle is enhanced following the topical injection of IGF- Ⅰ. This study was to evaluate the histology change of extraocular muscle after direct injection of IGF- Ⅰ . Methods The superior rectus muscle from lateral eyes in 7 normal adult rabbits received a single-time injection of 10μg （0.05 mL）IGF- Ⅰ , and the superior rectus muscle from fellow eye received an injection of 0.05 mL saline only. One week after injection,the morphology of the muscle both in IGF treated and control muscles was studied under the light microscope, and the activated satellite cells in superior rectus muscle were determined by immunohistochemistry. Results No obvious infiltration of inflammatory cells was seen in the muscles after injection. The perimeter of the orbital layer fibers in the superior rectus muscle was （ 120.24 ± 30. 76） μm in IGF-Ⅰinjection group and （ 108.48 ± 25.64 ） μm in control group, showing a significant difference between them（ P = 0. 000）. The perimeter of the global layer fibers in the superior rectus muscle was （178.99 ± 58.63 ）μm in IGF- Ⅰ injection group compared with control group （ 184.80 ± 55.59 ） μm without significant difference between them （ P = 0. 127）. The frequencies of large and small fibers in the muscles were more in IGF-Ⅰ injection group than that in control muscles （P = 0. 033 ）. The frequencies of activated satellite cells of both global and orbital layer in the muscles were more in IGF-Ⅰ injection group than those in control group（P = 0. 000）. Conclusion Direct injection of IGF- Ⅰ into extrocular muscles is an effective and safe procedure. IGF- Ⅰ promotes the proliferation of extraocular muscle fibers and activates the satellite cells, but the response of global and orbital layer muscle fibers to IGF- Ⅰ may be different.

关 键 词：眼外肌 胰岛素样生长因子Ⅰ 卫星细胞 

分 类 号：R777[医药卫生—眼科]