先天性牙根发育不良致病相关基因的筛选  

Screening candidate genes for congenital hypoplasia of teeth root.

作　　者：叶楠[1] 文玲英[2] 金岩[3] 方军[2] 杨富生[2] 卫克文[2] 金明[4] 轩昆[2] 陈嵘[1] 

机构地区：[1]解放军总医院第二附属医院口腔科,北京100091 [2]第四军医大学口腔医学院儿童口腔科 [3]第四军医大学口腔医学院组织病理学教研室 [4]第四军医大学生物化学与分子生物学教研室

出　　处：《临床口腔医学杂志》2009年第6期340-343,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金面上项目青年基金(30600709);陕西省科技计划-社发攻关项目2006k11-G1(2);第四军医大学口腔医学院创新工程项目资助

摘　　要：目的:筛选先天性牙根发育不良的致病相关基因。方法:采用改良消减杂交技术,以先天性牙根发育不良患儿及其正常兄弟为研究对象,制备血液基因表达cDNA文库,构建两者的差异表达文库,采用反向点杂交技术剔除假性克隆,挑取部分克隆进行定性测序。结果:构建的差异表达基因文库浓度为4.0×102,反向点杂交检测发现基因文库质量较好;获得了11个与先天性牙根发育不良疾病相关的基因克隆。结论:成功构建了先天性牙根发育不良致病相关基因的消减cDNA文库,推测筛选出的ADAM28和MCPR1可能是牙根发育不良的致病相关基因。Objective： To screen the candidate genes for congenital hypoplasia of teeth root disease. Method： By modified subtractive hybridization technique, we collected one boy with congenital hypoplasia of teeth roots and his healthy brother to obtain eDNA library of blood, then we constructed subtractive cDNA library. Furthermore, Pseudo-clones were removed by inverse clot blot hybridization, then some clones were picked up randomly for sequencing and analyzing. Result： The capacity of subtractive eDNA library was 4.0×10^2, and the library was identified to be suitable for further analysis by test of inverse dot blot hybridization. We obtained 11 genes associated with the disease of hypoplasia of teeth roots. Conclusion： We successfully constructed the subtractive cDNA library of candidate genes for congenital hypoplasia of teeth roots. Moreover we suggest that ADAM28 and MCPR1 genes should be responsible for this disease.

关 键 词：牙根发育不良 改良消减杂交技术 致病相关基因 

分 类 号：R780.2[医药卫生—口腔医学] Q786[医药卫生—临床医学]