实验性糖尿病牙周炎诱导成骨细胞凋亡的研究  

Osteoblast apoptosis in experimental diabetic periodontitis in rats

作　　者：付永伟[1] 和红兵[1] 欧炯光[1] 

机构地区：[1]昆明医学院附属口腔医院口腔内科,云南昆明650031

出　　处：《华西口腔医学杂志》2009年第3期252-255,259,共5页West China Journal of Stomatology

基　　金：云南省应用基础研究面上资助项目(2007c218m);昆明医学院第一附属医院院内基金资助项目(2004byf14)

摘　　要：目的观察糖尿病牙周炎条件下成骨细胞的凋亡情况。方法选用SD大鼠62只,随机分为糖尿病牙周炎组(DP)、单纯牙周炎组(P)以及空白对照组(N)。采用一次性腹腔注射链脲佐菌素(STZ)的方法诱导大鼠糖尿病模型,采用丝线结扎联合口内接种细菌的方法建立牙周炎模型。各组动物分别于DP组、P组丝线结扎后第3、6周时分批处死,取标本进行组织学检测,并计算各组成骨细胞凋亡百分率。结果丝线结扎后3、6周时,成骨细胞凋亡百分率由高至低依次为DP组、P组、N组,组间两两比较均有统计学意义(P<0.05)。在丝线结扎后3周和6周时,DP组成骨细胞凋亡百分率均达到P组的2倍。结论糖尿病可促进牙周炎条件下牙周组织中成骨细胞的凋亡。Objective To observe osteoblast apoptosis in diabetic periodontitis. Methods Sixty-two SD rats were randomly divided into three groups： Diabetic periodontitis group （DP）, periodontitis group （P） and normal control group （N）. Diabetes was induced by intraperitoneal injection of streptozotocin （STZ）. Periodontitis was initiated by ligating floss around maxillary second molars and inoculation of Porphyromonas gingivalis （P.gingivalis）. Three or six weeks later, the animals were sacrificed and the specimens were prepared for histological analysis. The percentage of apoptotic osteoblasts was examined. Statistical significance was determined by one-way ANOVA. Results The sequence of percentage of apoptotic osteoblasts among each group was group DP 〉group P 〉group N. Intergroup comparisons demonstrated that diabetic periodontitis and periodontitis might increase apoptosis of osteoblasts. The percentage of apoptotic osteoblasts in group DP was approximately two times higher than that in group P. Conclusion The present study clearly demonstrates that diabetes can increase the apoptosis of osteoblasts in periodontitis.

关 键 词：牙周炎 糖尿病 成骨细胞 凋亡 

分 类 号：R781.4[医药卫生—口腔医学]