超声振荡对于模拟根管内毒素的灭活作用研究  

Endotoxin deactivation in artificial glass root canals with ultrasonic treatment

作　　者：刘桂香[1,2] 王青[1] 苏林旺[1] 

机构地区：[1]山东大学口腔医院牙体牙髓科,山东济南250012 [2]山东大学齐鲁医院口腔科,山东济南250012

出　　处：《华西口腔医学杂志》2009年第3期280-282,共3页West China Journal of Stomatology

基　　金：山东省卫生厅基金资助项目(2001CALCAB15)

摘　　要：目的探讨超声振荡对于模拟根管内毒素的灭活作用。方法80个无热原玻璃单根管模型随机分为A～H8组,每组10个,分别注入标准内毒素溶液并给予不同处理:A、B、C组分别给予超声振荡5、7、10min;D组加入过氧化氢溶液并超声振荡5min,E组单纯加入过氧化氢溶液;F组加入次氯酸钠溶液并超声振荡5min,G组单纯加入次氯酸钠溶液;H组为对照组。采用动态浊度法鲎试验测定各组内毒素活性,分析各组间内毒素活性的差异。结果A、B、C组间以及与对照组之间内毒素活性的差异均无统计学意义(P>0.05);D、E、F、G组与对照组之间内毒素活性的差异均有统计学意义(P<0.001),4个实验组明显低于对照组;单纯药物组与药物加超声组之间内毒素活性的差异无统计学意义(P>0.05)。结论在本实验条件下,采用口腔科超声根管治疗仪进行单纯超声振荡,不能灭活感染根管内毒素,亦不能加强根管冲洗液灭活内毒素的作用。Objective To study the deactivation of the endotoxin in artificial glass root canals with ultrasonic vibration. Methods 80 artificial glass root canals were randomly divided into 8 groups： Ultrasonic vibration of 5 minutes, 7 minutes, 10 minutes, ultrasonic vibration of 5 minutes together with 3% H2O2 solution, only 3% H2O2 solution, ultrasonic vibration of 5 minutes together with 5.25% NaCIO solution, only 5.25% NaCIO solution and the control. Standard endotoxin solution was introduced into each root canal. Different time＇s ultrasonic vibration was applied to different groups. After ultrasonic vibration, the endotoxin activity of each group was tested by kinetic turbidimetric limulus test. Result There were no significant differences among the groups of different time ultrasonic vibration and the control （P〉0.05）. There was great statistical difference between each group with root canal rinse solution and the control （P〈0.001）. The endotoxin activity of the test was significantly lower than the control. There was no significant difference between the groups of only rinse solution and rinse solution together with ultrasonic vibration. Conclusion Under the condition of this study, the only ultrasonic vibration can not deactivate the endotoxin of infected root canals and can not intensify the effect of root canal rinse solution.

关 键 词：慢性根尖周炎 根管预备 超声冲洗 内毒素 动态浊度法鲎试验 

分 类 号：R781.05[医药卫生—口腔医学]