兔VX2舌鳞癌移植瘤模型3种建立方法的比较  

A comparison of three methods in establishing transplanted model of VX_2 tongue carcinoma in rabbits

作　　者：田军[1] 廖贵清[1] 刘海潮[1] 李传真[1] 李清[1] 王敬旭[1] 杜日昌 张同韩[1] 

机构地区：[1]中山大学附属口腔医院口腔颌面外科,广东广州510055 [2]广东省韶关市第一人民医院病理科,广东韶关512000

出　　处：《华西口腔医学杂志》2009年第3期326-329,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30171019);广东省自然科学基金资助项目(990089)

摘　　要：目的采用3种不同移植瘤方法建立稳定的兔VX2舌鳞癌移植瘤模型,并对这3种方法建立的模型的生物学特性进行比较。方法将72只新西兰大白兔随机分成3组,每组24只,分别用瘤组织块植入法、改良瘤细胞悬液注入法及瘤细胞悬液注入法植于兔舌侧缘中1/3黏膜下,建立兔舌鳞癌的移植瘤模型。观察和比较3种方法所建立的模型的肿瘤生长状况、成瘤率和自发转移发生率。结果瘤组织块植入组、改良瘤细胞悬液注入组、瘤细胞悬液注入组的成瘤率分别为83.3%、91.7%、33.3%,局部淋巴结转移率分别为71.4%、100.0%、37.5%,肺转移率分别为35.7%、81.3%、0;3种方法建立的兔VX2舌鳞癌移植瘤模型的组织学形态均符合中分化鳞状细胞癌特征。结论3种方法建立的移植瘤模型均较接近人舌鳞癌自然生长过程。其中,改良瘤细胞悬液注入法建立的动物模型,因成瘤率高、转移率高、同期瘤体大小差异较小,更适合于舌鳞癌研究。Objective To establish transplanted models of VX2 tongue carcinoma in rabbits by three methods and compare these models. Methods After establishment of VX2 tumor-bearing rabbits, 72 New-Zealand white rabbits were randomly divided into 3 groups. Intact tumour tissue, modified tumour cell suspension, tumour cell suspension were respectively injected into the middle-third lateral border of the tongues of rabbits in 3 groups to induce transplanted VX2 tongue carcinoma. The histological features, the tumour-take rates and the metastasis rates of the 3 models were observed. Results The tumour-take rate of 3 models were 83.3%, 91.7% and 33.3% respectively; the lymph node metastasis rates were 71.4%, 100.0% and 37.5% respectively; the lung metastasis rates were 35.7%, 81.3% and 0 respectively. The histological features of the transplanted VX2 tongue carcinoma of 3 models were all consistent with those of moderately differentiated carcinoma. Conclusion The biological properties of the transplanted VX2 tongue carcinoma of 3 models is much alike to tongue carcinoma in humans. The model established with modified tumor cell suspension is considered to be more suitable for tongue cancer study.

关 键 词：舌癌 VX2肿瘤细胞株 动物模型 

分 类 号：R739.8[医药卫生—肿瘤]