前列腺癌微环境中树突状细胞分布及功能状态的初步研究  

The Distribution and Functional Status of Dendritic Cells in the Microenviroment of Prostate Cancer

作　　者：齐士勇[1] 徐勇[2] 畅继武[2] 刘冉录[2] 杨阔[2] 李凯[1] 于大海[1] 王克明[1] 

机构地区：[1]天津市第三中心医院,300170 [2]天津医科大学第二医院

出　　处：《天津医药》2009年第6期433-435,共3页Tianjin Medical Journal

基　　金：天津市科技计划项目(项目编号:07ZCGYSF01000)

摘　　要：目的:探讨前列腺癌微环境中树突状细胞(DC)分布、功能状态的变化及其与记忆性T细胞的关系。方法:选取40例前列腺增生(BPH),40例前列腺上皮内瘤(PIN)及46例前列腺癌(Pca)石蜡组织标本,用S100标记组织中总DC、CD83标记成熟DC、CD45RO标记组织中记忆性T细胞,用SP法行免疫组化染色;观察阳性细胞分布,计算阳性细胞浸润密度,分析DC与T细胞的相关性。结果:由BPH、PIN到Pca的S100+DC、CD45RO+T浸润密度逐渐降低,前列腺癌组织降低明显(P<0.01);Pca中CD83+DC浸润密度高于BPH和PIN(P<0.05)。前列腺癌组织中,S100+DC主要分布在肿瘤间质(P<0.01),CD83+DC主要分布在癌上皮间及癌周(P<0.01),且CD83+DC与CD45RO+T浸润密度呈正相关(rs=0.831,P<0.05)。结论:前列腺癌组织中,DC数量减少且存在功能缺陷,不同功能状态的DC存在区域性分布;成熟DC数量的减少可能导致T细胞免疫功能下降。Objective：To investigate the distribution and functional status of dendritic cells （DC）in the microenvironment of prostate cancer, and its relationship with memory T lymphocyte. Methods：Forty benign prostatic hyperplasia （BPH）,40 prostatic intraepithelial neoplasm （PIN）and 46 prostate cancer （Pca） specimens were selected for immunohistochemistry （SP method）. The infiltrating density of the S100＋DC, CD83 ＋DC and CD45RO ＋T lymphocyte were calculated, meanwhile their distribution were observed. The relationship between DC and T lymphocyte was statistically compared. Results： The infiltrating density of S100＋DC and CD45RO＋T lymphocyte decreased gradually from BPH, PIN to Pca, especially in prostate cancer （P 〈 0.01）. While, in prostate cancer, the infiltrating density of CD83＋DC was more than that of BPH and PIN （P 〈 0.05）. S100＋DC mainly localized in the tumor stroma （P 〈 0.01）. CD83＋DC mainly presented in the tumor epithelium and advancing tumor margin （P 〈 0.01 ）and directly contacted with tumor cells. There was a significant correlation between the infiltrating density of CD83＋DC and T lymphocytes in Pca （rs=0.831 ,P 〈 0.05）. Conclusion： The number of DC decreased in prostate cancer. The different DC had different distributions in Pca and the lack of activated DC may decrease the function of T lymphocyte.

关 键 词：前列腺肿瘤 癌 树突细胞 S100蛋白质类 抗原CD 抗原CD45 

分 类 号：R737.25[医药卫生—肿瘤] R392.12[医药卫生—临床医学]