高原红细胞增多症动物模型的建立及其生理功能反应  

Establishment of an animal model of high altitude polycythemia and analyses of its physiological functional responses to hypoxia

作　　者：靳国恩[1] 韵海霞[1] 马兰[1] 白振中[1] 杨应忠[1] 曹越[1] 格日力[1] 

机构地区：[1]青海大学医学院高原医学研究中心,青海省西宁市810001

出　　处：《中国组织工程研究与临床康复》2009年第24期4713-4716,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30660052)~~

摘　　要：背景:国内外关于高原红细胞增多症的动物研究主要以模拟海拔进行,少数情况下进行现场研究,但在现场究竟多长时间可以使平原大鼠发生高原红细胞增多症还需要进一步研究。目的:建立高原红细胞增多症动物模型并对相关生理功能反应进行初步观察。设计、时间及地点:随机对照动物实验,于2007-08/09在青海大学医学院高原医学研究中心教育部和青海省重点实验室完成。材料:SPF级Wistar大鼠50只,体质量160～200g,雌雄各半。10只高原鼠兔(Pika)于青海玛多县4300m现场捕获,体质量100～180g。促红细胞生成素试剂盒由美国R&DSystems公司提供。方法:将30只Wistar大鼠随机分成高原15和30d两组(雌雄分开),每组各15只,运至海拔4300m青海省果洛州玛多县饲养。余20只大鼠为对照组在西宁海拔2260m饲养。另10只高原鼠兔作为高海拔对照组。分别在第15天和第30天进行血红蛋白、红细胞压积、促红细胞生成素测定。主要观察指标:①高原15d组和高原30d组中出现高原红细胞增多症阳性数。②高原红细胞增多症组与各正常对照组血红蛋白等指标的比较。③高原红细胞增多症组与各正常组促红细胞生成素水平的变化。④高原红细胞增多症组血红蛋白水平与血氧饱和度和促红细胞生成素相关性分析。结果:高原15,30d组大鼠血红蛋白,红细胞压积水平明显升高,与高海拔和低海拔对照组相比差异有显著性意义(P<0.01);高原15d对照组中血红蛋白>207g/L,红细胞压积>65%的高原红细胞增多症大鼠模型占75%,高原30d组中占93%。高原组促红细胞生成素值显著高于高海拔和低海拔对照组(P<0.01)。结论:通过对Wistar大鼠各项生理指标的测定认为在海拔4300m地区饲养30d才可完全建立高原红细胞增多症的动物模型,并进一步说明低海拔动物在不同海拔具有不同的生理反应机制。BACKGROUND： Most present animal studies on high altitude potycythemia, both at home and abroad, are taken under the circumstance of simulated altitude rather than on the real plateau. Therefore, further studies are still needed to determine how long it will take for plain rats to get this disease at the real high altitude. OBJECTIVE： To establish animal models with high altitude polycythemia and preliminarily analyze their physiological functional responses to hypoxia. DESIGH, TIME AND SETTING： A randomized control animal experiment was completed in the Research Center for High Altitude Medicine in Medical College of Qinghai University （the Key Lab of National Ministry of Education and Qinghai Province） from August to September in 2007. MATERIALS： A total of 50 Wistar rats of SPF grade were used, half male and half female, weighting 160 g to 200 g. Ten plateau pikas were captured at 4 300 m altitude in Maduo County, Qinghai Province, China. Eythropoietin （EPO） kit was offered by R＆D Systems Company, USA. METHODS： Of all the 50 Wistar rats, 30 ones were randomly divided into a high altitude 15-day group （15-day group） and a high altitude 30-day group （30-day group）, with 15 rats （of both genders） in each group. Then they were moved to 4 300 m altitude in Maduo County, Guoluo Prefecture, Qinghai Province, China, and were fed there for 15 and 30 days respectively. The other 20 ones were fed, as the low altitude control group, at 2 260 m altitude in Xining, Qinghai Province, China. The 10 pikas were classified as the high altitude control group. The hemoglobin （Hb）, haematocrit （Hct） and serum EPO level of all rats was determined at days 15 and 30 following feeding. MAIN OUTCOME MEASURES： （1）The number of rats who presented with high altitude polycythemia in the 15-day and the 30-day group. （2）The comparison of Hb levels between rats with high altitude polycythemia and those in the two control groups. （3）The comparison of serum EPO levels between rats with high al

关 键 词：高原红细胞增多症 模型 动物 生理功能 

分 类 号：R965.1[医药卫生—药理学]