γ射线对人肺癌A549细胞和人淋巴细胞Cx43基因转录表达的影响  

Effect of γ-rays on transcription of Cx43 gene in human lung cancer A549 and lymph cells

作　　者：李伟[1] 江其生[1] 郭丽杰[1] 李峰生[1] 宋秀军[1] 陈舒[1] 何蕊[1] 于惠杰[1] 

机构地区：[1]第二炮兵总医院中心实验室,北京100088

出　　处：《中华肿瘤防治杂志》2009年第10期726-728,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30271479)

摘　　要：目的:研究γ射线对人肺癌A549细胞和淋巴细胞Cx43基因转录表达的影响。方法:对数生长期的肺癌A549细胞和外周血淋巴细胞,分别给予1、2、3、5和6Gy的60Coγ射线照射,并于照射前(对照组)和照射后4、8、12、24、36、48和72h分别提取总RNA,反转录成cDNA。应用实时荧光定量PCR技术,检测各时相点Cx43基因表达改变。结果:低剂量1～6Gy照射后12h,人外周血淋巴细胞Cx43mRNA表达水平明显增高,P<0.05。肺癌A549细胞照射后Cx43mRNA表达水平(在1、3和6Gy照射12h后分别为0.06、0.12和0.09)与对照组(1.00)相比明显降低,P<0.05。结论:γ射线照射后能上调人淋巴细胞Cx43mRNA表达和下调肺癌A549Cx43mRNA表达,其机制可能与细胞类型及对γ射线的反应性有关。OBJECTIVE： To study the effect of γ-rays on the transcription of Cx43 gene in human lung cancer A549 and peripheral blood lymph cells. METHODS： Logarithmic growing phase of lung cancer cells A549 and lymph cells were irradiated under 1,2,3,5 and 6 Gy, respectively by γ-rays and RNA were extracted before irradiation and at 4,8,12,24,36,48 and 72 h after irradiation. Fluorescent quantitative PCR was used to detect the expression of Cx43 mRNA. RESULTS： The level of Cx43 mRNA in lymph cells was increased significantly by the dose of 1-6 Gy irradiation after 12 h（P〈0. 05）. In lung cancer A549 cells after irradiation, the level expression of Cx43 mRNA was 0.06, 0.12, and 0.09 fold under 1, 3, and 6 Gy at 12 h after irradiation, and the control group expression level was 1.00. The level expression of Cx43 mRNA was significantly lower than that of controls （P〈0. 05）. CONCLUSION： ）＇rays can upregulate the expression of Cx43 mRNA in human lymph cells and downregulate the expression of Cx43 mRNA in human tung cancer ceils A549, and its mechanism might be concerned with the type of cells and reactivity to γ-rays.

关 键 词：连接蛋白类 Γ射线 荧光 聚合酶链反应 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]