肺癌组织SKP2和p53蛋白表达临床意义的探讨  

Expressions of SKP2 and p53 proteins and their clinical significance in lung carcinoma tissues

作　　者：赵君[1] 丁卫忠[2] 刘景义[2] 王鹏航 柳致平 张欢[3] 杨春鹿[1] 

机构地区：[1]中国医科大学附属第一医院胸外科,辽宁沈阳110001 [2]沈阳市胸科医院胸外科,辽宁沈阳110044 [3]天津市塘沽区妇幼保健院超声中心,天津300451

出　　处：《中华肿瘤防治杂志》2009年第10期748-751,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨SKP2、p53蛋白在肺癌组织中的表达和关系及其对预后的影响。方法:组织芯片和免疫组化技术检测SKP2、p53蛋白在89例非小细胞肺癌(NSCLC)、13例小细胞肺癌(SCLC)和10例肺良性病变组织中的表达。结果:NSCLC、SCLC及肺良性病变组织中SKP2和p53蛋白的表达差异均有统计学意义,P值均为0.000。SKP2蛋白在肺癌组织中的表达水平与细胞分化程度、病理类型、TNM分期及淋巴转移密切相关,P值均为0.000。p53蛋白在肺癌组织中的表达水平与细胞分化程度、病理类型密切相关(P值均为0.000)。肺癌组织中SKP2、p53蛋白表达呈正相关(r=0.448,P=0.000),两者同时高表达与细胞分化程度及病理类型密切相关(P值分别为0.001和0.022)。多因素分析显示,肺癌患者5年生存率与SKP2蛋白表达水平、TNM分期、淋巴转移及术后放/化疗密切相关。结论:SKP2和p53蛋白在肺癌的发生发展中可能存在某种协同作用,对肺癌患者的预后可能有一定影响。OBJECTIVE： To research the expressive characteristics of SKP2 and p53 proteins in lung carcinoma and their affection to patients＇ prognosis. METHODS： The expressions of SKP2 and p53 proteins were detected in 89 NSCLC, 13 SCLC, and 10 lung benign lesion tissues by the tissue chip and immunohistochemistry technology. RESULTS： The positive rates of SKP2 and p53 protein stain were significantly different among NSCLC, SCLC and lung benign lesion tissues （P =0. 000）. The expressive level of SKP2 protein in lung carcinoma tissues was closely related to the cell differentiation, pathological type, TNM stage and lymph node metastasis （P=0. 000）. The expressive level of p53 protein in lung carcinoma tissues was closely related to the cell differentiation and pathological type （P= 0. 000）. The expression of SKP2 protein was positive correlation with p53 protein in lung carcinoma tissues （r= 0. 448, P=0. 000）. The rate of concurrent high expression of them was closely related to the cell differentiation and pathological type （P= 0. 001 ; P=0. 022）. The multiplicity displayed that the lung carcinoma patients＇ 5 years survival rate was closely related to the expressive level of SKP2 protein,TNM stage, lymph node metastasis,postoperative radiotherapy and chemotherapy. CONCLUSIONS： SKP2 and p53 proteins may play some conspiracy in occurrence and development of lung carcinoma. Moreover, they may affect lung carcinoma patients＇ prognosis.

关 键 词：肺肿瘤 S期激酶相关蛋白质类 蛋白质P53 蛋白质阵列分析 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]