非小细胞肺癌组织COX-2和p53表达及其临床意义的探讨  

Expressions of COX-2 and p53 and their clinical significance in non-small cell lung cancer tissues

作　　者：杨锡贵[1] 张学德[2] 崔立献 

机构地区：[1]山东省肿瘤医院内科,山东济南250117 [2]章丘市人民医院肿瘤科,山东章丘250200

出　　处：《中华肿瘤防治杂志》2009年第10期752-754,763,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨COX-2和突变性p53蛋白在非小细胞肺癌(NSCLC)发生、发展中的作用机制及两者之间的相关性。方法:流式细胞仪检测80例NSCLC组织和20例癌旁正常肺组织中COX-2蛋白、突变型p53蛋白的表达情况。结果:NSCLC组织中COX-2蛋白较正常组织高表达,P<0.01。COX-2表达与有无淋巴结转移(P=0.002)和肿瘤大小(P=0.045)有关,与NSCLC患者性别、TNM分期、分化程度和组织学类型无关,P>0.05。NSCLC组织中p53蛋白较正常组织高表达,P<0.01。p53蛋白表达与有无淋巴结转移(P=0.01)有关;而与NSCLC患者性别、肿瘤的大小、TNM分期、肿瘤分化程度和组织学类型无关,P>0.05。NSCLC组织中p53和COX-2蛋白同时阳性表达率为47.82%(22/46),p53阴性COX-2蛋白阳性表达率为58.82%(20/34),两者表达无相关性,P=0.33。结论:COX-2的过表达及p53蛋白突变与NSCLC的发生发展有关,两者之间无相关性。OBJECTIVE： To investigate the expressions of COX-2 and p53 in human non-small cell lung cancer tissues and their correlation with biological behaviors of non-small cell lung cancer. METHODS： The expressions of COX-2 protein and mutation p53 protein were detected by flow cytometry in tissues of 80 eases of non-small cell lung cancer and 20 normal tissues adjacent to cancer. RESULTS： A high level of COX-2 protein was expressed in non-small cell lung cancer tissues compared with the normal tissues （P〈0. 01）. The expression of COX- 2 was significantly correlated with the lymph node metastasis （P = 0. 002） and tumor size （P=0. 045）, but not with their sexes and TNM stages, tumor grade, histologic type （P〉0. 05）. A high level of p53 protein was expressed in non-small cell lung cancer tissues compared with the normal tissues, P〈0. 01. The expression of p53 was significantly correlated with the lymph node metastasis （P=0. 01）, but not with their sexes and TNM stages, tumor size tumor grade, and histologic type （P〉0. 05）. The positive rate of p53 and COX-2 was 47. 82%（22/46）, and the positive rate of COX-2 with negative expression of p53 was 58. 82% （20/34）, P=0. 33. CONCLUSION： COX-2 over expresson and p53 mutantion are correlated with the genesis and progress of non-small cell lung cancer, but there is no significant correla- tion between COX-2 protein and p53 protein.

关 键 词：癌 非小细胞肺 前列腺素内过氧化物合酶 蛋白质P53 流式细胞术 

分 类 号：R734.2[医药卫生—肿瘤]