松材线虫与拟松材线虫分泌的纤维素酶系研究  

Study on Cellulase and Dispersal Ability from Bursaphelenchus xylophilus and B. mucronatus

作　　者：马海宾[1,2] 梁军[1] 吕全[1] 张星耀[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室,北京100091 [2]中国林业科学研究院热带林业研究所,广东广州510520

出　　处：《林业科学研究》2009年第3期397-400,共4页Forest Research

基　　金：国家“十一五”科技支撑项目(2006BAD08A191);科技部国际合作项目(2007DFB30270);国家重点基础研究发展计划(2002CB111404)

摘　　要：采用羧甲基纤维素钠(CMC)、微晶纤维素(MC)和水杨素(SC)三种底物,用刚果红染色法和DNS法分别定性和定量分析了松材线虫和拟松材线虫分泌物中纤维素酶系组分和酶活力。结果表明,松材线虫和拟松材线虫分泌物中存在完全降解纤维素所需的3种酶系组分:内切-β-1,4-葡聚糖酶、外切-β-1,4-葡聚糖酶和β-葡萄糖苷酶,松材线虫分泌物中三种酶活力明显高于拟松材线虫酶活力。研究结果为更好的解释松材线虫致病机理提供了科学数据。The secretion cellulase of Bursaphelenchus xylophilus and B. mucronatus was analyzed while CMC, MC and SC were used to be the celllose resouces for the nematodes secretion degrading. The ceUulase activities were tested with staining and DNS methods from Bursaphelenchus xylophilus and B. mucronatus. The results showed that there were 3 cellulase component in both B. xylophilus and B. mucronatus secretion： endo-β-1,4-glucanase,exo-β-1, 4-glucanase and β-glucosidase. The cellulase activity from B. xylophilus was higher than that from B. mucronatus. It was inferred that the pathogenicity of B. xylophilus was more serious than that of B. mucronatus for the reason of the cellulase activity.

关 键 词：松材线虫 拟松材线虫 纤维素酶系 酶活力 

分 类 号：S763[农业科学—森林保护学]