CoQ10对DA损伤PC12细胞保护作用的实验研究  

Experimental study on the protective effect of CoQ10 on damaged PC12 cells induced by DA

作　　者：李熙东[1] 刘兴 隋汝波[1] 赵宝东[3] 

机构地区：[1]辽宁医学院附属第一医院神经内科,辽宁锦州121001 [2]锦州市红十字血站,辽宁锦州121000 [3]辽宁医学院解剖学教研室,辽宁锦州121000

出　　处：《中国医药导报》2009年第18期28-30,共3页China Medical Herald

摘　　要：目的:探讨辅酶Q10(CoQ10)在以PC12细胞建立的多巴胺神经元损伤细胞模型中的作用。方法:实验于2007年4月～2008年4月在辽宁医学院科技实验中心进行。在体外培养PC12细胞的条件下,加入多巴胺以损伤PC12细胞,同时加入一定浓度的CoQ10,检测细胞的生长活力及培养液MDA含量,比较多巴胺损伤的PC12细胞在加与不加CoQ10时损伤的差异。结果:CoQ10+多巴胺组PC12死亡率及MDA含量较多巴胺组显著降低(P<0.05)。结论:CoQ10能减轻多巴胺对PC12细胞的损害。Objective： To discuss the influence of CoQIO on the model of dopaminergic neuron based on PC12 cells. Methods： The experiment was done in the experimental center of science and technology of Liaoning Medical College from April 2007 to April 2008. DA was used to damage the cultured PC12 cells and a certain concentration of CoQ10 was added in the medium used to culture PC12 cell in vitro. The level of MDA in PC12 cells and the cell growth vigor were measured to compare the difference between the damaged PC12 cells by DA with and without CoQ10. Results： The mortal- ity rate and the level of MDA in CoQ10 and DA group were remarkably decreased compared with that of DA group （P〈 0.05）. Conclusion： CoQ10 can reduce the injury of PC12 cells induced by DA.

关 键 词：CoQ10 大鼠肾上腺嗜铬细胞瘤细胞 帕金森病 细胞凋亡 

分 类 号：R742.5[医药卫生—神经病学与精神病学]