京巴犬α-干扰素全基因的克隆与分子进化分析  

Cloning and Phylogenetic Analysis of Interferon-α Gene of Jingba Canine

作　　者：陈红英[1,2] 李新生[1] 董海聚[1] 崔保安[1,2] 郭明彦[1] 宋亚朋[1,2] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省动物性食品安全重点实验室,河南郑州450002

出　　处：《江西农业大学学报》2009年第3期512-516,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家"十一五"科技支撑计划专项(2006BAD06A08)

摘　　要：根据基因库(GenBank)中收录的犬α-干扰素(IFN-α)基因核苷酸序列(AB102731),设计并合成了1对引物,PCR扩增京巴犬IFN-α全基因,并克隆、测序。结果表明,犬IFN-α基因全基因序列为564 bp,包含1个开放阅读框,编码187个氨基酸的多肽,推导氨基酸序列有3个潜在的N-糖基化位点,有10个与二硫键形成有关的半胱氨酸。序列比较分析发现,京巴犬IFN-α基因序列与GenBank发表的其它品种犬IFN-α基因序列核苷酸同源性为96.3%以上,氨基酸同源性为94.1%以上,其中与AB102731核苷酸同源性最高,为99.5%,仅有3个碱基差异。京巴犬与人和其他8种动物的IFN-α基因序列分析和系统进化分析表明,IFN-α基因存在种属的差异性,亲缘关系越近,同源性越高。京巴犬IFN-α基因的成功克隆为进一步研究犬IFN-α基因表达、生物学活性和应用奠定基础。One pair of primers was designed and synthesized according to the canine interferon alpha gene （IFN- α） nucleic acid sequence （AB102731） published in the GenBank. Jingba canine IFN-α gene was amplified by PCR, then cloned and sequenced. The results showed ： cloned canine IFN - α gene span was 564 bp, which included one open - reading frame, encoding 187 amino acid residues, three potential N - glyeo- salation sites and ten cysteines in the deduced amino acid sequence. The results of homology analysis with other canine interferon - alpha gene published previously in the GenBank by DNAStar analysis revealed that there was identity of 96.3% at nucleotide homology and that of 94.1% at amino acid homology. The results of comparative sequence analysis and phylogenetic tree analysis indicated ： there was difference of genus in IFN -α gene, the nearer relationship, the higher homology. This study paved the way for future study of biological function and application of canine IFN- α.

关 键 词：京巴犬 α-干扰素基因 扩增 基因克隆 进化分析 

分 类 号：Q789[生物学—分子生物学]