中药复方对小鼠肺癌细胞增殖和转移的影响  

EFFECT OF TRADITIONAL CHINESE MEDICINE COMPOUND ON PROLIFERATION AND METASTASIS OF LUNG CANCER CELL

作　　者：张维彬[1] 梁朝晖[1] 胡少为[1] 杨海峰[1] 

机构地区：[1]广东省中医院病理科,广州510120

出　　处：《现代预防医学》2009年第12期2332-2333,2336,共3页Modern Preventive Medicine

基　　金：广东省中医药管理局科研基金资助(98335)

摘　　要：[目的]研究扶正祛邪中药复方抑制小鼠肺癌细胞增殖及转移的分子机制。[方法]构建小鼠Lewis肺癌模型,应用免疫组化方法检测中药复方、替加氟和生理盐水对各组肿瘤细胞Ki67和CD44V6表达的情况,并记数各组小鼠肺组织内肿瘤转移结节的数量,比较各组间的差异。[结果]生理盐水组的Ki67及CD44V6阳性表达率显著高于中药复方组和替加氟组(P﹤0.05),而后两者差异无统计学意义(P﹥0.05);中药复方治疗组和替加氟组小鼠肺内转移瘤结节数量少于生理盐水组,差异有统计学意义(P﹤0.05),中药复方治疗组结节数量多于替加氟组,差异无统计学意义(P﹥0.05)。[结论]中药复方能有效降低小鼠肺癌细胞Ki67及CD44V6阳性表达率,抑制癌细胞增殖和肺内侵袭转移。[ Objective] To detect the molecular mechanism of traditional Chinese medicine compound （TCMC） and pathogenic faetor eliminating herbal medicine inhibiting proliferation and metastasis of lung cancer cell. [ Methods] Mouse models with Lewis＇ s lung cancer were constructed. The immunohistoehemical method was used to examine the expressions of Ki67 and CD44 V6 proteins in tumor cells treated by traditional TCMC, tegafur and normal saline. In additional, the number of tumor metastatic loci in the lungs of different groups was counted and the difference among them was Compared. [Results] The positive rates of Ki67 and CD44V6 expression in normal saline group were significantly higher than those in TCMC and tegafur groups （P 〈 0.05）, but there was no significant difference between TCMC and tegafur groups （P 〉 0.05）. The number of tumor metastatic loci in the lungs of TCMC and tegafur groups was significantly lower than that in normal saline group, respectively （P 〈 0.05 ）. The number of tumor metastatic foci in the lungs of TCMC group was more than that in tegafur group, but there was no significant difference between them （P 〉 0.05）. [ Conclusion] TCMC could effectively reduce the expressions of Ki67 and CD44V6 proteins and inhibit the proliferation and metastasis of lung cancer cell.

关 键 词：肺癌 中药复方 肿瘤增殖抗原(Ki67) CD44V6 

分 类 号：R734.2[医药卫生—肿瘤]