人脑胶质瘤中BMI1的表达及其意义  

Expression of BMIl in Human Gliomas and Its Significance

作　　者：朱爱华[1] 陆华[1] 肖新如[2] 赵理[3] 陈涛[3] 刘伟[3] 彭小忠[3] 袁建刚[3] 赵继宗[2] 

机构地区：[1]无锡市第三人民医院神经外科,江苏省无锡市214041 [2]北京天坛医院神经外科 [3]北京协和医科大学基础研究所

出　　处：《中国肿瘤临床》2009年第11期640-643,共4页Chinese Journal of Clinical Oncology

基　　金：国家863计划基金资助(编号:2002BA711A08)~~

摘　　要：目的:研究基因BMI1在中枢神经系统最常见的肿瘤-胶质瘤组织中的表达情况,并探讨其表达水平与胶质瘤的恶性程度及发生、发展的关系。方法:使用荧光定量实时PCR技术检测基因BMI1在34例各级别胶质瘤手术切除组织及10例正常对照组织中的表达。同时对34例各级别胶质瘤组织及10例正常对照脑组织使用免疫组化的方法检测BMI1蛋白的表达。结果:实时PCR的结果显示,基因BMI1在对照脑组织、Ⅱ级胶质瘤、Ⅲ级胶质瘤及Ⅳ,级胶质瘤组织中的表达相对值分别为0.655、2.65、5.62及5.43。胶质瘤组中BMI1的表达水平显著高于对照组(P=0.001).恶性胶质瘤组(Ⅲ级与Ⅳ级胶质瘤)中BMI1的表达水平显著高于良性胶质瘤(Ⅱ级胶质瘤),JP=0.015,Ⅲ级胶质瘤组与Ⅳ级胶质瘤组之间未发现存在差异(P=0.902);免疫组化结果显示,BMI1蛋白在对照脑组织、Ⅱ级胶质瘤、Ⅲ级胶质瘤和Ⅳ级胶质瘤组织中的表达阳性率分别为10%、25%、100%和100%。各级别胶质瘤组织中BMI1蛋白的阳性表达率显著高于正常脑组织对照组(P=0.001),而且在恶性胶质瘤组织中BMI1蛋白的阳性表达率显著高于良性胶质瘤(P=0.000)。结论:无论是在mRNA水平还是在蛋白水平上,BMI1在各级别脑胶质瘤标本中的表达水平均明显高于正常脑组织对照,且恶性胶质瘤中BMI1的表达要明显高于良性胶质瘤。BMI1在胶质瘤的发生、发展过程中有重要作用。Objective： To investigate the expression level of BMI1 in human glioma, the most common tumor in the central nervous system, and to explore the relationship between BMI1 expression and malignant degree, tumorigenesis, and progression of human glioma. Methods： The expression of BMI1 mRNA was detected in 34 tumor samples and 10 normal samples using real-time PCR. The expression of BMI1 protein was detected with immunohistochemistry using sections from the same samples. Results： The results of real-time PCR demonstrated that the expression ratio of BMI1 mRNA was 0.655, 2.65, 5.62 and 5.43 in normal control tissues, grade Ⅱ, Ⅲ, and Ⅳ brain glioma, respectively. The expression level of BMI1 mRNA was significantly higher in human glioma than in the normal control tissues （P〈0.01）. There was also a significant difference （P〈 0.05） in BMI1 mRNA expression level between the malignant glioma （Grades Ⅲ and Ⅳ） and benign glioma （Grade Ⅱ）. There was no statistically significant difference between Grade Ⅲ glioma and Grade Ⅳ glioma （P〉 0.05）. The results of immunohistochemistry illustrated that BMI1 protein was expressed in 10%, 25%, 100% and 100% of the normal control tissues, grade Ⅱ, Ⅲ, and Ⅳ brain glioma, respectively. A significant difference was found in the expression of BMI1 protein between 34 glioma tissues and the normal samples （P〈 0.05）. There was a significant difference in the expression of BMI1 protein between the malignant glioma （Grade Ⅲ and Ⅳ glioma） and benign glioma （Grade Ⅱ glioma）. Conclusion： The BMI1 expression at both the mRNA and protein levels was significantly higher in glioma than in normal control tissues. The expression level of BMI1 in malignant glioma is significantly higher than that in benign glioma. The BMI1 gene plays an important role in the tumorigenesis and progression of human glioma.

关 键 词：胶质瘤BMI1 实时PCR免疫组化 多梳家族 

分 类 号：R739.4[医药卫生—肿瘤]