肝X受体激动剂对ApoE基因敲除小鼠主动脉壁C-反应蛋白和CD40配体表达及平滑肌细胞含量的影响  

Effects of liver X receptor agonist on the expressions of C-reactive protein and CD40 ligand and contents of smooth muscle cells in the aorta of ApoE gene knockout mice

作　　者：程军[1] 李金平[1] 田卓[1] 胡厚源[1] 

机构地区：[1]第三军医大学西南医院心血管内科,重庆400038

出　　处：《第三军医大学学报》2009年第13期1281-1283,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30770851)~~

摘　　要：目的探讨肝X受体(liver X receptor,LXR)激动剂T0901317对高脂饲养ApoE基因敲除(apolipoprotein E gene knockout,ApoE-/-)小鼠在动脉粥样硬化病变形成的早期动脉壁内C-反应蛋白(CRP)和CD40配体(CD40L)表达及平滑肌细胞含量的影响。方法8周龄雄性ApoE-/-小鼠12只,按随机数字表法分入LXR激动剂T0901317组和二甲基亚砜(DMSO)溶剂对照组,每组6只。均给予高脂饲养8周,在高脂饲养的后4周,分别给予LXR激动剂T090131720mg·kg-1·d-1或相当剂量的DMSO腹腔注射。麻醉处死小鼠后,取小鼠主动脉,以石蜡包埋,行主动脉根部连续切片,采用免疫组化法检测主动脉壁内CRP、CD40L和平滑肌细胞α-actin的表达,以Image Pro Plus 6.0软件进行图像分析。结果LXR激动剂组动脉壁CRP表达水平较对照组明显减少(P<0.05),LXR激动剂组动脉壁CD40L表达水平较对照组明显减少(P<0.05),动脉粥样硬化斑块内平滑肌细胞α-actin表达水平与对照组比较没有统计学差异(P>0.05)。结论LXR激动剂可能通过抑制ApoE-/-小鼠动脉壁中CRP和CD40L的表达,减轻血管壁的炎症反应,从而发挥抗动脉粥样硬化形成的作用。Objective To investigate the effects of liver X receptor agonist on the expressions of C-reactive protein and CD40 ligand and smooth muscle cell α-actin in the aorta of ApoE gene knockout mice with earlier atherosclerosis. Methods Male ApoE gene knockout mice （8-week old） were divided randomly into control group and T0901317 treatment group （n = 6 in each group）. The mice in T0901317 group were administered intraperitoneally with T0901317 at the dose of 20 mg·kg^-1·d^-1 for 4 weeks. Mice in the control group were only given equivalent amount of dimethyl sulfoxide （DMSO）. The expressions of C-reactive protein and CD40 ligand and smooth muscle cell α-actin were detected by immunological histochemical method. Results The expressions of C-reactive protein and CD40 ligand in the atherosclerotic plaque in the aortic wall were significantly lower in T0901317 group as compared with those in the DMSO control group （P 〈 0.01 and P 〈 0.05 ）. No difference was found in the content of smooth muscle cell α-actin in the atherosclerotic plaque （P 〉 0.05 ）. Conclusion Liver X receptor agonist may reduce the formation of atherosclerotic lesions by inhibiting the in-flammation and the expressions of C-reactive protein and CD40 ligand in the aorta of ApoE gene knockout mice.

关 键 词：肝X受体 APOE基因敲除小鼠 C-反应蛋白 CD40配体 平滑肌细胞 炎症 

分 类 号：R-332[医药卫生] R543.5