^(99)Tc^m-EGFR-McAb及^(99)Tc^m-CD44-McAb单独及联合用于荷人肺腺癌裸鼠的显像研究  

Effects of radioimmunoimaging with ^(99)Tc^m-EGFR-McAb or ^(99)Tc^m-CD44-McAb or combined application of both on nude mice bearing human lung adenocarcinoma

作　　者：段东[1] 李少林[2] 朱玉泉[1] 王树斌[2] 

机构地区：[1]重庆医科大学附属第一医院核医学科,重庆400016 [2]重庆医科大学基础医学院核医学教研室,重庆400016

出　　处：《第三军医大学学报》2009年第13期1287-1290,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30570523)~~

摘　　要：目的评价99Tcm-EGFR-McAb及99Tcm-CD44-McAb单独及联合应用于荷人肺腺癌裸鼠的放射免疫显像效果。方法建立荷人肺腺癌裸鼠动物模型;采用直接法99Tcm标记抗EGFR和CD44的单克隆抗体,运用柱层析法对标记抗体分离、纯化,并对标记、纯化后的抗体特性进行鉴定;利用99Tcm-EGFR-McAb及99Tcm-CD44-McAb单独及联合进行荷人肺腺癌裸鼠显像及体内分布研究。结果99Tcm对EGFR-McAb和CD44-McAb的标记率分别为(91.5±3.8)%、(92.3±4.1)%,标记抗体比活度分别为(2.8±0.3)MBq/μl、(2.9±0.5)MBq/μl,放射化学纯度分别为(96.5±2.8)%、(96.2±3.1)%。放射免疫显像结果显示,肿瘤组织对2种标记抗体均有较高的摄取,2种标记抗体联合使用肿瘤部位的放射性浓聚明显高于2种标记抗体单独使用,通过ROI技术测得T/NT值分别为(5.58±0.46)、(2.72±0.22)、(2.30±0.18)。标记抗体的体内分布规律与显像结果一致。结论99Tcm-EGFR-McAb、99Tcm-CD44-McAb用于荷人肺腺癌裸鼠的放射免疫显像能得到较理想的靶/非靶比值,2种标记抗体的联合应用,可在一定程度上提高靶/非靶比值,优于一种标记抗体的单独使用。Objective To evaluate the effects of radioimmunoimaging with ^99Tc^m-EGFR-McAb or ^99Tc^m-CD44-McAb or combined application of both on nude mice bearing lung adenocarcinoma. Methods Animal model of nude mice bearing lung adenocarcinoma was established. The direct labeling method of ^99Tc^m was applied to labeling monoclonal antibodies for EGFR and CD44, and then the properties of the labeling antibodies purified by SephadexG50 Column were identified. The radio-immuno-image as well as body distribution concerning nude mice bearing lung adenocarcinoma was studied by application of ^99Tcm-EGFR-McAb or ^99Tcm-CD44-McAb alone or combined application of both. Results The labeling rates of ^99Tc^m for EGFR-McAb and CD44-McAb were （91.5±3.8 ）% and （92.3±4.1 ）% respectively. Specific activities of the labeled antibod- ies were （2.8±0.3） MBq/g and （2.9±0.5 ） MBq/g, respectively. The rates of radiochemical purity were （96.5±2.8） % and （96.2±3.1 ） %, respectively. The tumor tissue had high intake of the two labeled antibodies according to radioimmunoimaging result. The radioactivity concentration of the combined application of the two labeled antibodies was obviously higher than that of the single application. The T/NT relative values measured through ROI technique were 5.58±0.46, 2.72±0.22, and 2.30±0.18, respectively. The body distribution result of the labeled antibodies and their imaging results were basically identical. Conclusion The optimal target and non-target ratios could be obtained by application of ^99Tc^m-EGFR-McAb or ^99Tc^m- CD44- McAb alone in nude mice bearing lung adenocarcinoma. The target and non-target ratios could be enhanced through the combined application of the two antibodies.

关 键 词：肿瘤 表皮生长因子受体 CD44 抗体 单克隆 锝 放射性核素显像 

分 类 号：R730.45[医药卫生—肿瘤] R734.2[医药卫生—临床医学]