清洁级SD大鼠血液学指标及生化指标正常值的大样本分析  

Analysis for the normal range of hematological and serum biochemical parameters in healthy CL SD rat based on large sample

作　　者：李罡[1] 刘建勋[2] 葛争艳[2] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院生理教研室,沈阳110016 [2]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《中药药理与临床》2009年第2期128-131,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:建立本实验研究中心SD大鼠血液学指标和生化指标的正常值范围,并为进一步制定全国统一标准提供参考依据。方法:选用清洁级(CL,cleananimal)健康SD大鼠(雄性170只,体重为116±24g;雌性170只,体重为107±22g)。腹主动脉采血,测定了血液学指标和血清生化指标。然后对测定结果使用SPSSV13.0的"两独立样本T检验功能(Independent-Samples T Test)"进行了统计分析(雄性动物与雌性动物视为两独立样本),并讨论并分析了性别差异。结果:得出了雄性及雌性SD大鼠血液学指标正常值和血清生化指标正常值的均值,以及标准差。性别间比较显示:对于部分血液学指标和血清生化指标,不同性别SD大鼠的正常参考值存在明显差异(P<0.05)。结论:所有血液学及血液生化指标的样本内集中趋势明显,其算术均值对相应指标的整体水平具有代表性。另外,白细胞总数(WBC)、血红蛋白(HGB)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、总蛋白(TP)、白蛋白(ALB)、尿素氮(BUN)等8项指标的正常参考值存在明显的性别差异(P<0.05)。Objective： To establish the normal range of hematological and serum biochemical parameters in SD rats for our research center and provide references for the establishment of the country standard. Methods： We have CL SD rats as investigative object（ male： n = 170, body weight 116 ± 24g ; female： n = 170, body weight 107 ± 22g）. Blood sample was collected from abdominal aorta at the end of the experiment. At same time, both hematological and serum biochemical parameters were mensurated. Then we used the ＂ Independent-Samples T Test＂ process of SPSS V 13.0 to analyze the acquired data（ male and female were regarded as two independent samples）, and difference between sexes was also analyzed. Results： Through analysis, we acquired the mean and the standard deviation of both hematological and serum biochemical parameters of beth male and female SD rats. Comparison between sexes showed： referring to some hematological and biochemical parameters, there was apparent difference between sexes （ P 〈 0.05 ）. Conclusion： All the hematological and serum biochemical parameters has apparent concentrating trend, and thus their arithmetic means can represent the integral level of corresponding parameters. In addition, the 8 biochemical parameters of WBC, HGB, ALT, AST, ALP, TP , ALB, BUN manifest apparent difference between sexes （ P 〈 0.05 ）.

关 键 词：SD大鼠 血液学指标 血清生化指标 

分 类 号：R-332[医药卫生] R563.1