Lims A:一个新的LIM同源域基因的克隆和初步分析  

Lims A:molecular cloning and characterization of a novel splice variant of the LIM domain-family gene

作　　者：陈曦[1] 赵沐子[2] 李建民[2] 刘庆淮[1] 

机构地区：[1]南京医科大学第一附属医院眼科,中国江苏省南京市210029 [2]南京医科大学细胞生物学与医学遗传学系,中国江苏省南京市210029

出　　处：《国际眼科杂志》2009年第6期1036-1040,共5页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30000088)~~

摘　　要：目的:克隆与分析大鼠不同剪切的Lims基因。方法:应用巢式RT-PCR,以SD大鼠cDNA为模板,扩增Lims基因不同剪切子,构入PinPointTMXa-1T质粒,测序鉴定。结果:测序表明克隆了新的Lims基因变异剪切体Lims A,该变异剪切体编码区为1014bp,编码338个氨基酸。结论:比较基因组学分析显示,成功地克隆了一新的大鼠Lims基因剪切子Lims A,为进一步探索Lims基因在增生性玻璃体视网膜病变中的功能奠定基础。AIM： To clone and analyze the different splicing variant of Lims gene in rat. METHODS： Splicing variants of Liras gene were amplified by RT-PCR from rat cDNA and inserted into PinPoint^TM Xa-IT vector, the position clones selected by PCR were sequenced. RESULTS： Lims A, a novel splicing variant with a 1 014bp open reading frame （ORF）, encoding a 338-amino acid （AA） protein was cloned. CONCLUSION： Comparative genome analysis displayed Lims A, a novel variant of Lims gene was confirmed in rat and established the foundation for further to studying the function of Liras A in proliferative vitreoretinopathy.

关 键 词：Lims基因 基因表达 基因剪切 增生性玻璃体视网膜病变 

分 类 号：R774.1[医药卫生—眼科]