视网膜色素上皮损伤后相邻感光细胞及脉络膜血管的病理变化  

Histopathological changes of photoreceptors and choroids after retinal pigment epithelium debridements

作　　者：张陶然[1] 胡运韬[2] 李颖[2] 吴建国[2] 王艳玲[1] 马志中[2] 

机构地区：[1]首都医科大学附属北京友谊医院眼科,中国北京市100050 [2]北京大学眼科中心,中国北京市100083

出　　处：《国际眼科杂志》2009年第6期1041-1045,共5页International Eye Science

基　　金：中国国家重点基础研究发展规划资助项目("973"项目)(No.2007CB512203);中国北京大学第三医院重点临床项目(No.YLZD09-6-04)~~

摘　　要：目的:研究视网膜色素上皮(retinal pigment epithelium,RPE)损伤后相邻感光细胞和脉络膜血管的病理变化。方法:机械性刷除青紫蓝兔眼底髓线上方的RPE细胞,观察术后7,14,30d和90d清除区相邻感光细胞和脉络膜血管变化。结果:RPE细胞清除术后相邻的感光细胞发生退行性改变和死亡,脉络膜毛细血管萎缩。结论:RPE清除术可模拟萎缩型年龄相关性黄斑变性(age-related macular degeneration,AMD)及渗出型AMD剥膜以后的病理变化,为自体RPE移植挽救损伤的感光细胞的实验提供了良好的动物模型。AIM： To create a retinal pigment epithelium（RPE） debridement animal model and observe the histopathological changes. METHODS： Abrasive debridement of RPE was performed with a metal cannula after superior retinal bleb detachments in rabbits. Animals were examined by fundus fluorescein angiography （FFA） and indocyanine green angiography（ICGA） in seriate 7 days, 14 days, 1 month and 3 months post-debridements. And tissue sections were evaluated using light microscope and transmission electron microscope at corresponding time points. RESULTS： The debrided area was not completely resurfaced by surrounding elongated RPE cells; the choriocapillaries beneath the debrided area underwent degeneration; and the photoreceptors overlying the debrided area showed gradually degeneration and loss. CONCLUSION： The type of abrasive debridement has been suggested as a model of therapy for macular degeneration as there is a high correlation between RPE dropout, choriocapillaries atrophy and loss of photoreceptors function.

关 键 词：视网膜色素上皮 感光细胞 脉络膜毛细血管 黄斑变性 

分 类 号：R779.1[医药卫生—眼科]