阳离子脂质体介导人小梁细胞基因转染实验研究  

Liposome mediated gene transfer into human trabecular meshwork cells

作　　者：毛晓春[1] 黄刚[1] 张少维[1] 王根国[1] 

机构地区：[1]华中科技大学同济医学院附属襄樊医院(襄樊市中心医院)眼科,中国湖北省襄樊市441021

出　　处：《国际眼科杂志》2009年第6期1056-1057,共2页International Eye Science

摘　　要：目的:观察脂质体是否能介导目的基因转移至人小梁细胞及脂质体潜在的毒副作用。方法:通过体外细胞转染实验优化阳离子脂质体与质粒DNA的比例,荧光显微镜观察目的基因的表达。结果:小梁细胞内可见脂质体介导的绿色荧光蛋白表达,高浓度脂质体导致细胞死亡和脱落。结论:阳离子脂质体可有效介导目的基因转移至小梁细胞内。但高浓度对正常的细胞有一定的毒副作用。AIM： To evaluate the efficiency of liposome mediated gene transfer to trabecular endothelial cells and cells damage caused by liposome. METHODS： The ratio of cationic liposome to green fluorescein protein （GFP） expression plasmid for high efficacy of transfection was tested in vitro. The GFP expression was observed by using fluorescence microscopy. RESULTS： The GFP expression in trabecular endothelial cells was observed, However , high concentration liposome caused the death and falling off of trabecular endothelial cells. CONCLUSION： Liposome seems to be able to deliver genes to trabecular endothelial cells with efficacy, but high concentration liposome used here is to some extent harmful to corneal endothelial cell.

关 键 词：小梁细胞 脂质体 转染 

分 类 号：R775[医药卫生—眼科]