经方侯氏黑散对脑缺血损伤大鼠神经可塑性相关蛋白的影响  

Mechanism of Hou Shi Hei San in Accelerating Repair of Nerve after Cerebral Ischemia in Rats

作　　者：张秋霞[1] 赵晖[1] 王蕾[1] 崔海[1] 穆阳[1] 

机构地区：[1]首都医科大学中医药学院中医临床基础教研室

出　　处：《首都医科大学学报》2009年第3期341-346,共6页Journal of Capital Medical University

基　　金：北京市教育委员会科技发展计划(KM2003100025099)资助项目~~

摘　　要：目的观察经方侯氏黑散对大脑中动脉闭塞大鼠模型缺血脑组织神经生长相关蛋白43(growth-associated protein-43,GAP-43)、微管相关蛋白2(microtubule-associated protein-2,MAP-2)、突触体素(synatophysin,SYN)表达的影响,探讨其促进脑缺血后大鼠神经功能康复的机制。方法用线栓法阻断大脑中动脉,建立大鼠局灶性脑缺血再灌注损伤模型,用免疫组化与图像分析相结合的方法观察缺血脑组织的轴突、树突、胞体以及突触损伤及修复情况。结果模型组大鼠MAP-2免疫阳性树突稀疏,SYN免疫反应物明显减低,仅能看到散在点状物,与对照组比较差异有统计学意义(P<0.05)。侯氏黑散组大鼠海马CA1区、CA3区GAP-43表达面积和表达强度均明显高于模型组,差异有统计学意义(P<0.05)。侯氏黑散组大鼠MAP-2和SYN积分光密度值均明显高于模型组,差异有统计学意义(P<0.05)。结论经方侯氏黑散能够上调GAP-43、MAP-2以及SYN的表达,可有效诱导脑损伤修复过程中神经元结构蛋白的合成,促进神经再生和神经功能的康复。Objective To probe into the mechanism of Hou Shi Hei San in accelerating repair of nerve after cerebral ischemia in rats by observing the changes of growth-associated protein-43 （ GAP-43 ）, microtubule-associated protein-2 （ MAP-2 ） and synatophysin （SYN） in the tissue of cerebral ischemia. Methods Middle cerebral artery occlusion model was replicated by string ligation. Using behavioral test, the authors valuated the neurological deficiency of each group after operation. The injury and repair of neuron, dentrite and axon were observed by immunohistochemistry combined with image pattern analysis 15 days after the rat models were made. Results There were obvious disturbances of nerve function, scarce dentrite of MAP-2 positive cell and immunoreaction of SYN reduced in the model group. The grade of injuries of nerve function in the group treated with Hou Shi Hei San was lower than that in the model group （P 〈 0.05）. The area and intensity of expression of GAP-43 in CAI and CA3 areas of hippocampi in treatment groups were significantly higher than that in model group （ P 〈 0.05 ）. The integrated optical density （IOD） values of MAP-2 and SYN expressions markedly increased in treatment groups （ P 〈 0.05 ）. Conclusion These results suggest that Hou Shi Hei San can increase the expressions of GAP- 43, MAP-2 and SYN and promotes nerve regeneration through inducing the synthesis of construction protein of neuron effectively, thus accelerates the repair of nerve.

关 键 词：侯氏黑散 脑缺血 神经修复 

分 类 号：R743[医药卫生—神经病学与精神病学]