应用微阵列芯片分析人髓母细胞瘤及瘤旁组织miRNA表达谱差异  

Microarray-based miRNAs Profiling in Human Medulloblastomas

作　　者：刘巍[1] 晁腾飞[2] 龚燕华[2] 袁建刚[2] 甲戈[1] 马振宇[1] 赵继宗[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经外科 [2]中国医学科学院北京协和医学院基础医学研究所医学分子生物学与国家重点实验室

出　　处：《首都医科大学学报》2009年第3期355-359,共5页Journal of Capital Medical University

基　　金：国家高技术研究发展计划(2002BA711A08)资助项目~~

摘　　要：目的检测人髓母细胞瘤(medulloblastomas,MB)与非肿瘤脑组织中微小RNA(miRNA)的表达差异情况,探讨异常表达miRNA参与MB发生的可能分子机制。方法选取经手术切除的MB组织标本及瘤旁正常小脑组织,分别提取总RNA和小分子RNA,在miRNA微阵列芯片中杂交检测,通过芯片扫描和数据分析,并通过实时荧光定量RT-PCR验证,获得该病异常miRNAs表达谱。结果筛选出9个MB的相关miRNAs,其中表达上调4个,表达下调5个。其中miR-17、miR-100、miR-106b和miR-218的差异表达情况得到定量RT-PCR的证实。结论miRNA微阵列分析技术是一种快速、高效的研究生物信息的分子生物学技术;筛选出的差异表达miRNAs可能参与MB发病,为此病诊治提供了新的思路,值得进一步研究。Objective To screen the differentially expressed microRNAs （miRNAs） in medulloblastomas （MB） using miRNA microarray and to explore the relationship between pathogenesis and miRNAs aberrantly expressed in MB. Methods The tumor tissue MB and normal cerebellar tissue adjacent to MB tumors were obtained from sporadic MB undergoing surgical resection. Total RNA, including the small RNAs, was extracted and isolated respectively. Hybridization was carried out on miRNA microarray chip. Real-time quantitative RT- PCR was performed to confirm results obtained by microarray analysis. Results Nine miRNAs have been identified, 4 of them were upregulated and the remaining five were down-regulated in cancer tissues. The differential expression of m/R-17, miR-100 and m/R-106b and m/R-218 was confirmed by microarray analysis. Conclusion MiRNA microarray analysis is a high-throughput, comprehensive molecular biological technology. The aberrant expression of miRNA genes in this study may be involved in the pathogenesis of human MBs.

关 键 词：髓母细胞瘤 微小RNA 微阵列 靶基因 肿瘤发生 

分 类 号：R739.42[医药卫生—肿瘤] Q786[医药卫生—临床医学]