Notch基因在人乳腺癌中的作用  

Notch gene in human breast cancer

作　　者：花本林[1] 付欣鸽[2] 尹亮[1] 李锋[1] 

机构地区：[1]石河子大学医学院病理学教研室,新疆石河子832002 [2]汕头大学医学院第一附属医院病理科,广东汕头515041

出　　处：《世界肿瘤杂志》2009年第2期99-103,共5页Tumour Journal of the World

基　　金：国家自然科学基金课题（NO.30760072）;2008年度广东省医学科研基金（卫生厅）课题（NO.A2008442）

摘　　要：目的检测Notch1和JAG1在乳腺癌中的表达，及其与乳腺癌相关临床指标的关系，分析Notch基因在人类乳腺癌中的作用和意义。方法应用逆转录聚合酶链反应（RT—PCR）检测60例乳腺癌组织和25例癌旁正常乳腺组织Notch1和JAG1的表达，对乳腺癌组织与癌旁组织进行表达率和表达强度标准化系数的统计学比较，并在不同的腋窝淋巴结转移情况间、不同TNM分期和病理学分级间进行Notch1表达强度标准化系数的统计学比较。结果乳腺癌组织Notch1的表达率和标准化系数分别为93．3％（56／60）和0．83，均明显高于癌旁组织，乳腺癌组织中JAG1的表达率为10％（6／60），癌旁组织中无JAG1表达，伴腋窝淋巴结转移的病例Notch1标准化系数高于无腋窝淋巴结转移的病例；乳腺癌Ⅰ期病例Notch1标准化系数（0．57）低于Ⅱ期（1．05）Ⅱ期高于Ⅲ期（0．59），Ⅰ期与Ⅲ期差异无统计学意义；乳腺癌Ⅰ级病例Notch1标准化系数（0．55）低于Ⅱ级（0．83）和Ⅱ级低于Ⅲ级（1．05），Notch1可能在分化较好的人乳腺癌中的表达是低的，在分化较差的人乳腺癌中的表达是增高的。结论人类乳腺癌中存在Notch1和JAG1的异常高表达，提示Notch1的异常表达与活化可能与人类乳腺癌的形成有关，在人类乳腺癌不同发展阶段的作用可能不同。Objective To explore the role of Notch gene in human breast cancers, the expression of Notchl and its ligand JAG 1 in human breast cancers and their relationships with clinical stages of breast cancers were analyzed. Methods RT-PCR was used to detect the expression of Notchl and JAG1 in 60 breast cancer specimens and 25 normal breast tissues at the margin of tumor sections, and the statistical difference of expression rates and standardized coefficient between the two groups were analyzed. To compare the expression intensity of Notchl and JAG1 at the different TNM stages and pathological grades in the illness, and at the different stages with or without axillary node metastasis. Results The expression rate and standardized coefficient of Notchl in human breast cancers were significantly higher than those of normal breast tissues at the margin of tumor sections. The expression rate of JAG1 in human breast cancers was 10%, while JAG1 was not detected in normal breast tissues at the margin of tumor sections. The standardized coefficient of Notchl in cases with axillary node metastasis was significantly higher than that in cases withoutaxillary node metastasis. The standardized coefficient of Notchl at stage Ⅰ was significantly lower than that at stage Ⅱ, and stage Ⅱwas significantly higher than stage Ⅲ There was no statistically significant difference between stage Ⅰ and stage Ⅲ. The standardized coefficient of Notchl at grade Ⅰ was significantly lower than that at grade Ⅱ, and grade Ⅱ was significantly lower than grade Ⅲ. Notchl maybe expression was low in highly-differentiated tumors and increased in poorly-differentiated tumors. Conclusions Notchl and JAG1 are highly expressed in human breast cancers, indicating that the aberrant expression and activation of Notchl may be related to tumorigenesis of human breast cancer. Notchl may play different roles at different development stages of human breast cancer.

关 键 词：NOTCH 基因 信号通路 乳腺肿瘤 

分 类 号：R737.9[医药卫生—肿瘤] R73-34[医药卫生—临床医学]