细胞角蛋白19-mRNA在口腔鳞癌侵袭转移中表达的意义  

Correlation of expression of cytokeratin 19-mRNA with invasion and metastasis in oral squamous cell carcinoma

作　　者：舒萍[1] 鲁大鹏[1] 

机构地区：[1]首都医科大学口腔医学院,北京2005级硕士研究生100050

出　　处：《北京口腔医学》2009年第3期128-131,共4页Beijing Journal of Stomatology

基　　金：北京市留学人员科技活动择优资助市优秀项目;北京市卫生局留学回国人员科研专项经费项目;教育部留学回国人员科研启动基金资(教外司留〔2004〕176号)

摘　　要：目的探讨单层细胞角蛋白CK19mRNA的表达与口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)的局部侵袭与转移的关系。方法选用23例OSCC组织及相应癌旁组织,通过半定量RT-PCR方法观察OSCC中CK19-mRNA的表达,探讨其与肿瘤临床分期、分化程度、淋巴结转移的关系。检测CK19-mRNA在OSCC患者外周血中的表达,探讨其与OSCC远处转移及复发的关系。结果23例OSCC组织中CK19-mRNA均呈阳性表达,且表达量明显高于癌周组织(P<0.01)。CK19-mRNA表达仅与分化程度有关。23例OSCC手术前后外周血中均无CK19-mRNA的表达。结论CK19-mRNA可作为辅助诊断OSCC分化程度的指标,其表达水平的改变与OSCC转移无关,不能作为判断OSCC远处转移的指标。Objective To investigate the relationship between expression of CK19 and invasion and metastasis of oral squamous cell carcinoma. Methods Twenty-three patients with oral squamous cell carcinoma were investigated for the expression of CK19-mRNA by semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR）. Correlations between clinical stages, pathological differentiation, lymphatic metastasis and the expression of CK19-mRNA were evaluated. CK19-mRNA expression in peripheral blood of these patients and 23 healthy people were also examined. During follow-up after operation,the peripheral blood was collected again for the expression of CK19-mRNA. Results Expression of CK19-mRNA was found in all patients, but the expression level was different. The expression of CK19-mRNA was only associated with tumor differentiation. CK19-mRNA was negative in all of 23 blood specimens before operation and during follow-up. Conclusion CK19-mRNA is correlated with tumor differentiation of OSCC and not associated with tumor metastasis.

关 键 词：口腔鳞状细胞癌 细胞角蛋白19 转移 

分 类 号：R739.85[医药卫生—肿瘤]