产L-丝氨酸菌株SYPS-062的鉴定及碳源对发酵的影响  

Identification of L-Serine Producer SYPS-062 and the Effect of Different Carbon Source

作　　者：张晓梅 窦文芳 许泓瑜 许正宏[1,2] 

机构地区：[1]江南大学医药学院制药工程研究室,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《微生物学通报》2009年第6期789-793,共5页Microbiology China

基　　金：江苏省博士后基金(NoSY20061019);国家973计划项目(No2007CB707804);国家863计划项目(No2006AA020104);教育部新世纪优秀人才支持计划(NoNCET-07-0380)

摘　　要：采用形态学、生理生化实验和16SrDNA序列分析的方法对从自然界中筛选得到的一株能直接利用糖质原料发酵生产L-丝氨酸菌株SYPS-062的分类地位进行了研究,确定其为谷氨酸棒杆菌(Corynebacterium glutamicum)。同时考察了碳源对菌株SYPS-062发酵产L-丝氨酸的影响,实验结果表明,当蔗糖浓度为60g/L时,菌株SYPS-062生物量和L-丝氨酸的积累均达到最大值,分别为8.1g/L和6.6g/L。SYPS-062 was an L-serine producing strain stored by our lab, which could directly produce L-serine from sugar. According to morphology, physiological and biochemical identification and 16S rDNA sequence, SYPS-062 was identified as Corynebacterium glutamicum. And the effect of different carbon source for the SYPS-062 and Corynebacterium glutamicum ATCC13032 fermentation were studied. The experiment result showed that the sucrose was 60 g/L, the yield of L-serine and biomass reached the maximal value, 6.6 g/L and 8.1 g/L respectively.

关 键 词：L-丝氨酸 谷氨酸棒杆菌 鉴定 糖质原料 直接发酵 

分 类 号：Q93[生物学—微生物学]