植物NADP^+-依赖型异柠檬酸脱氢酶的分子进化及功能研究进展  

Advances in molecular evolution and functions of NADP^+-dependent isocitrate dehydrogenase in higher plants

作　　者：王鹏[1,2] 赵旵军[1,2] 翟羽佳[1,2] 朱国萍[1,2] 

机构地区：[1]安徽师范大学生物大分子进化重点实验室 [2]安徽师范大学分子生物学及生物技术研究所,芜湖241000

出　　处：《生物学杂志》2009年第3期45-48,共4页Journal of Biology

基　　金：国家自然科学基金(30500300);教育部新世纪优秀人才支持计划(NCET-06-0558);安徽省优秀青年科技基金(06043089);安徽省引进海外留学人才基金(2005Z032)

摘　　要：高等植物NADP+-依赖型异柠檬酸脱氢酶(ICDHs)定位于细胞质、线粒体、叶绿体和过氧化物酶体等植物细胞的不同部位,由不同的基因编码,属于一个高度保守的多同工酶蛋白家族。对近年来关于植物NADP+-依赖型异柠檬酸脱氢酶的分子进化及功能研究进行综述,同时提出了未来植物ICDHs的研究重点和方向。最新的分子系统学分析显示,植物中不同细胞定位的ICDH同工酶聚在各自相应的进化枝上,动物或植物中不同细胞器的ICDH同工酶均来源于各自祖先ICDH基因的独立倍增。最新的功能研究表明,ICDHs催化合成的α-酮戊二酸可为植物细胞对氨的吸收同化提供碳骨架,而NADPH可以维系细胞内的氧化还原平衡,帮助植物抵御氧化胁迫。NADP^＋ -dependent isocitrate dehydrogenases （ICDHs） of the higher plant localize in the cytosol, mitochondria, chloroplast and peroxisome. ICDHs are encoded by different genes and belong to a multi-isoenzymatic family which is highly con- served. The advances in molecular evolution and functions of ICDHs in higher plant were summarized and some suggestions were put forward for future researches in this paper. The recent phylogeny analysis indicated that all plant ICDHs are located in a sepa- rate plant clade, and ICDH isoforrns have arisen through independent duplications of an ancestral ICDH gene within animal or plant kingdom. The recent investigation of functions implied that ICDHs of higher plants plays possible roles in the production of α-ketoglutarate for providing the carbon skeletons for plant ammonium assimilation and NADPH for maintaining the cellular redox balance for the defense of oxidative stress.

关 键 词：高等植物 异柠檬酸脱氢酶 NADP 分子进化 功能 

分 类 号：Q554.5[生物学—生物化学]