缺血预处理及后处理对家兔肾脏缺血-再灌注损伤的保护机制研究  

Protection of ischemic precondition and postcondition on renal ischemia injury in rabbit models

作　　者：郭志英[1] 任启伟[1] 王立赞[1] 王伟[1] 张德磊[1] 杨阁[1] 谭祥芹[1] 

机构地区：[1]济宁医学院基础学院,山东日照276826

出　　处：《济宁医学院学报》2009年第3期159-161,共3页Journal of Jining Medical University

摘　　要：目的探讨缺血预处理及缺血后处理对家兔肾脏缺血-再灌注损伤的保护作用。方法首先建立家兔肾脏缺血-再灌注模型。40只雄性家兔随机分为4组(n=10):假手术组(Sham组)、缺血再灌注组(I/R组)、缺血预处理组(IPC组)及缺血后处理组(IPO组)。再灌注60min后分别检测血清中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性,光镜下观察肾组织病理学改变。结果与Sham组比较,I/R组、IPC组和IPO组血清SOD活性降低,MDA含量升高,病理损伤明显;与I/R组相比,IPC组和IPO组血清SOD活性升高,MDA含量降低,病理损伤减轻。结论缺血预处理及后处理能够减轻家兔肾脏缺血-再灌注损伤,其机制与增强机体的抗氧化损伤能力有关。Objective To investigate protection of ischemic precondition（IPC）and postcondition（IPO）on renal ischemia reperfusion（I/R）injury in rabbits. Methods Firstly renal I/R injury models were used in this experiment. Forty male rabbits were randomly divided into 4 groups（n=10 each） ： group Ⅰ：sham operation, group Ⅱ：I/R injury,group Ⅲ： IPC, group Ⅳ： IPO. After reperfusion 60min, blood MDA content and SOD activity were measured.The changes of renal tissue were detected by microscope. Results SOD activity was significantly lower whereas the serum MDA content was significantly higher in I/R group,IPC group and IPO group than in sham group（P~ 0.05）. The serum SOD activity was significantly higher whereas MDA content was significantly lower in IPC group and IPO group than in I/R group. Microscopic examination showed that the renal I/R injury was significantly atten- uated in IPC group and IPO group. Conclusion IPC and IPO attenuate the renal injury induced by I/R in rabbits, and the protective effect is associated with the enhancement of the body antioxidation.

关 键 词：缺血预处理 缺血后处理 肾脏缺血 再灌注损伤 

分 类 号：R363.2[医药卫生—病理学]