高效液相色谱法测定地锦草中没食子酸的含量  

Determination of gallic acid in Herba Euphorbia humifusae by HPLC

作　　者：黄彩虹[1] 雷鹏[1] 甘莲莲[1] 黄义[1,2] 李新中[1] 

机构地区：[1]中南大学湘雅医院药剂科,长沙410008 [2]中南大学药学院,长沙410013

出　　处：《中南药学》2009年第6期408-410,共3页Central South Pharmacy

基　　金：湖南省卫生厅中医药科技基金项目(编号:6304-78);长沙市科技局重点项目(编号:K0802115-31)

摘　　要：目的建立高效液相色谱法测定地锦草中没食子酸的含量。方法DiamonsilC18色谱柱（4．6mm×250mm，5μm）；流动相：甲醇-0．01％磷酸（10：90）；检测波长：273nm柱温：30℃；流速：1mL·min^-1，进样量：5μL。结果没食子酸在0．0t82～0．1824μg线性关系良好，回归方程为Y=2969．4X-4．252，r=0．9999。平均加样回收率为98．36％，RSD为2．83％。结论本方法简便、快速、重复性好，可用于测定地锦草中没食子酸的含量。Objective To establish an HPLC method for the determination of gallic acid in Herba Euphorbia humifusac. Methods The chromatographic system consisted of a Diamonsil C18 column （4.6 mm ×250mm, 5 μm） and a mobile phase of MeOH-0.01%H3PO4 （10 ： 90）. The detection wavelength was 273 nm. The column temperature was 30 ℃ and the flow rate was 1 mL ·min^-1. Results A good linearity of gallic acid was in the range of 0. 018 2-0. 1824μg, the regression equation was Y= 2 969. 4 X--4. 252, and r was 0. 999 9. The average recovery was 98.36% and RSD was 2.83%. Conclusion The HPLC method is simple, rapid and accurate, which can be used for the determination of gallic acid in Herba Euphorbia humifusae.

关 键 词：地锦草 高效液相色谱法 没食子酸 

分 类 号：R927.2[医药卫生—药学]