响应面法优化多杀菌素发酵培养基的研究  

Response surface methodology for optimization of spinosad fermentation medium

作　　者：裘晓华[1] 裘娟萍[1] 张正波[1] 寿佳丽[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《工业微生物》2009年第3期21-25,共5页Industrial Microbiology

摘　　要：采用响应面分析方法,对刺糖多孢菌(Saccharopolyspora spinosa)H-2产多杀菌素的发酵培养基进行优化研究。运用单因子试验筛选出葡萄糖和棉籽粉为最适碳源和氮源,通过Plack-ett-Burman设计试验,对影响发酵培养基的8个相关因子进行评估并筛选出具有显著效应的4个因子:葡萄糖、棉籽粉、黄豆饼粉及玉米浆。通过最陡爬坡实验逼近以上4个因子的最大响应区域后,采用Box-Behnken响应面分析法,确定发酵产多杀茵素最佳培养基为葡萄糖64.5g,麦芽糖20g,玉米浆2g,大豆油40g,棉籽粉25g,黄豆饼粉2.4g,蛋白胨25g,CaCO35g,定容至1L,pH7.0。培养基优化后多杀菌素产量由278.1mg/L提高到508.7mg/L,比初始多杀茵素产量提高了1.83倍。Response Surface Methodology （RSM） was used to optimize fermentation medium for spinosad production by Saccharopolyspora spinosa H-2. In single factor experiment, glucose and Proflo cottonseed flour were screened out as the most suitable carbon and nitrogen sources. And Plackett-Burman design assay was used to evaluate eight factors related to spinosad production and four factors had statistically significant effects （glucose, Proflo cottonseed flour, soybean cake meal and corn steep liquor）. Then steepest ascent procedures were applied to define optimal response region for these four factors, finally the optimal factor concentrations were determined adopting RSM analysis. The results showed that glucose 64.5g/L, maltose 20g/L, corn steep liquor 2g/L, bean oil 40g/L, pmflo cottonseed flour 25g/L, soybean cake meal 2.4g/L, peptone 25g/L and CaCO35g/L combined the most suitable media for spinosad production, which increased the productivity of S. spinosa from 278. lmg/L to 508.7mg/L

关 键 词：多杀菌素 刺糖多孢菌 响应面法 优化 

分 类 号：TQ455[化学工程—农药化工] TQ920.6[轻工技术与工程—发酵工程]