Sulfolobus solfataticus P_2嗜热嗜酸α-淀粉酶在大肠杆菌和毕赤酵母中的表达  

Expression of thermophilic and acidophilic extracellular a-amylase gene from Sulfolobus solfataticus P_(2) in E. coli and Pichia pastoris

作　　者：高雅英[1] 沈标[1] 

机构地区：[1]南京农业大学生命科学学院/农业部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《工业微生物》2009年第3期56-61,共6页Industrial Microbiology

基　　金：科技部农业微生物菌种资源整理整合及共享试点(2005DKA21201-11)。

摘　　要：通过PCR方法从Sulfolobus solfataticus P2中扩增到2.6kb的α-淀粉酶基因(SSO1172),将其分别克隆到表达载体pET32a(+)和pPICZaA,并在E.coliRosetta和Pichia pas-toris GS115中进行表达。结果表明α-淀粉酶基因在Rosetta中得到了高效表达,酶活为143.466U/mL;而在GS115中表达量稍低,发酵液酶活力为98.102U/mL。A thermophilic and acidophilic extracellular α-amylase gene （S SO1172）, amplified by PCR from the genome of Sulfolobus solfataticus P2, was cloned into plasmid pET32a（ ＋ ）, pPICZaA and expressed in Escherichia coli Rosetta and Pichia pastoris GSl15 respectively. The results showed that a-amylase gene was highly expressed in Escherichia coli Rosettawith specific activity 143. 466 U/mL. The α-amylase could be expressed in Pichia pastoris GSl15 and secreted out with the activity of 98. 102 U/mL.

关 键 词：硫矿硫化叶菌 嗜热嗜酸α-淀粉酶 融合表达 

分 类 号：TQ925[轻工技术与工程—发酵工程]