杂交捕获法和PCR-RDB法检测妇女HPV感染的比较  

Comparison between HC2 and PCR-RDB for detecting HPV infection in women

作　　者：肖克林[1] 吴丽娟[1] 王敏[2] 郑有为[3] 钱靖琳[3] 王丁[3] 周克元[4] 

机构地区：[1]深圳市宝安区妇幼保健院检验科,518133 [2]中山大学达安基因股份有限公司科研部,广州510665 [3]广东省人民医院病理医学部,广州510080 [4]广东医学院生物化学与分子生物学教研室,广东湛江524023

出　　处：《国际检验医学杂志》2009年第6期545-547,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨HC2法和PCR-RDB法检测妇女HPV感染的异同点。方法利用HC2法和PCR RDB法检测妇女生殖道HPV感染状况,然后进行比较。结果81.14%(400/493)的样本PCR-RDB法和HC2法检测结果一致,两法一致性检验Kappa值为0.63(95%CI,0.57～0.69)。在HC2法RLU/CO<1.00、1.00～9.99、10.00～99.99、100.00～999.99和≥1 000.00的样本中,PCRRDB法13种高危型HPV的检出率依次为1.60%(3/187)、29.85%(20/67)、69.88%(58/83)、86.52%(77/89)和91.04%(61/67),五组间比较,差异有统计学意义(X^2=289.3,P<0.01)。HC2法高危型HPV试剂可与HPV53、66、11、cp8304等基因型发生交叉反应。结论HC2法和PCR-RDB法具有较好的一致性,HC2法存在交叉反应现象,PCR-RDB法难以标准化。Objective To compare hybrid capture-Ⅱ （HC2） to polymerase chain reaction （PCR）-reverse dot-blot（RDB） for detecting HPV infection in women. Methods HC2 method and PCR-RDB were applied to detecting human papillomaviral infection in female genital duct, and the test results were analyzed. Results Concordant results were found in 81.14% （400/493） samples （Kappa =0.63; 95% CI, 0. 57-0. 69）. The high risk type HPV positive rate detected by PCR-RDB were 1.60% （3/187）,29.85% （20/67）, 69.88% （58/83）, 86.52% （77/89） and 91.04% （61/67）, respectively, when the HC2 RLU/CO of samples were〈1. 00, 1.00-9.99, 10.00-99.99, 100.00- 999.99 and ≥1 000.00. Statistical differences were found among the five groups （Х^2 = 289.3, P〈0.01）. Cross reactions were found between HC2 high risk probe and HPV53, 66, 11, cp8304 and other genotypes. Conclusion There is good concordance between HC2 and PCR-RDB, though cross reaction exists in HC2. Relatively speaking, there are more influential factors for PCR-RDB.

关 键 词：人乳头瘤病毒16 人乳头瘤病毒18 聚合酶链反应 交叉反应 

分 类 号：R737.33[医药卫生—肿瘤] R450[医药卫生—临床医学]