根癌农杆菌介导的CMO基因转化马铃薯的研究  

Study on Potato Transformation of CMO Gene Mediated by Agrobacterium tumefaciens

作　　者：栗亮[1] 文钢[1] 杨涛[2] 张宁[2] 司怀军[1,2] 王蒂[1] 

机构地区：[1]甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《生物技术》2009年第3期16-19,共4页Biotechnology

基　　金：高等学校博士学科点专项科研基金项目(20050733003);国家高技术研究发展计划(863计划)项目(2006AA100107);甘肃省农业生物技术研究与应用开发项目(GNSW-2006-01)资助

摘　　要：通过根癌农杆菌介导法将强组成型表达启动子CaMV 35S和逆境诱导表达启动子rd29A驱动的菠菜胆碱单加氧酶(CMO)基因导入马铃薯栽培品种夏波蒂(Shepody)和费乌瑞它(Favorita)中,获得了抗卡那霉素再生植株,对转化植株进行PCR检测初步表明CMO基因已整合到转基因马铃薯基因组中。并对光照强度对马铃薯试管薯遗传转化的影响进行了研究,结果表明在诱导芽分化的过程中,2 000 lx的光照强度有利于试管薯薄片直接再生出抗性芽。Choline monooxygenase （CMO） gene driven by the constitutive promoter CaMV 35S and the stress induced promoter rd29A were introduced into two potato cuhivar Shepody and Favorita by Agrobacterium tumefaciens mediation. Putative transgenic plants rooting on kanamycin -containing medium were obtained, and PCR analysis preliminary showed that the CMO gene has been integrated into genome of the transformed potato. The result from effect of light intensity on transformation of potato micmtuber demonstrated that the light intensity of 2 000 lx was beneficial to resistant shoot formation directly from transformed micmtuber discs.

关 键 词：胆碱单加氧酶基因 马铃薯 根癌农杆菌 转化 光照强度 

分 类 号：Q785[生物学—分子生物学]