耐盐促生细菌Rs-198的鉴定及其与Rs-5混合培养研究  

Identification of Salt Tolerant Promoting Growth Bacteria Rs-198 and Study on Co-culture with Rs-5

作　　者：吴忠红[1,2] 马晶[3] 王芸 杨良[4] 娄恺 

机构地区：[1]新疆农业大学食品科学学院,新疆乌鲁木齐830052 [2]新疆特殊环境微生物实验室,新疆乌鲁木齐830091 [3]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [4]石河子大学食品学院,新疆石河子832003

出　　处：《生物技术》2009年第3期63-66,共4页Biotechnology

基　　金：新疆维吾尔自治区高技术研究发展计划项目("主要农作物生态耐盐促生剂的研制及其产业化示范";200811108)资助

摘　　要：目的:对具有耐盐促生作用的细菌Rs-198进行鉴定,并研究耐盐促生细菌Rs-198和Rs-5在混合培养中的相互作用。方法:利用菌株的形态特征、生理生化特征和16S rDNA序列同源性对菌株Rs-198进行鉴定;根据菌株Rs-5和Rs-198的拮抗共生性和生长周期特性确定混合培养温度;通过分析不同接种方式下单独培养和混合培养中葡萄糖的残余量、生物量的生成及pH的变化,揭示混合培养的相互作用。结果:菌株Rs-198初步鉴定为荧光假单孢菌(Pseudomonas fluorescens);Rs-198和Rs-5混合培养温度为28℃;不论按等体积混合,还是按浓度比为107∶106,106∶106,105∶106混合,两菌种均具有协同增长作用,当接种浓度相当时(106∶106),混合培养的活菌数是单独培养活菌数总和的3.1倍,此时协同增长作用最显著。结论:两菌株存在协同增长作用,为今后的混合培养和复合菌肥的研制提供了理论依据。Objective：Identification of salt tolerant Promoting Growth Bacteria Rs - 198,and Study on the interaction of Rs - 198 and Rs - 5 in co - culture. Method：Based on the morphological characteristics, physiological and biochemical characteristics, 16S rDNA sequence homology, the salt tolerant promoting growth bacteria Rs- 198 was identified; According to Antagonism and the growth cycle, the temperature of co-culture was determined; According to analyzing the glucose consumption, biomass production and pH change in mono- and co- culture, the interaction in co - culture was revealed. Result： Rs - 198 was identified as Pseudomonas fluorescens ; The temperature of co - culture was 28℃ ; Not only equivalent volume mixture but also different concentration ratios such as 107：106, 106：106, 10s ：106 ,there was synergistic growth efficacy between Rs 198 and Rs - 5. While the concentration was equivalent（ 10^6 ： 10^6 ）, tiffs efficacy was most obvious. Conclusion： There was synergistic growth efficacy between Rs - 198 and Rs - 5, it provided the theoretical foundation for co - culture of the two strains and preparation of biologically compound fertilizer in future.

关 键 词：鉴定 混合培养 发酵 荧光假单孢菌 

分 类 号：Q93-331[生物学—微生物学]