bcl-2基因表达变化在槲皮素抑制肝细胞癌生长中的作用  

Role of bcl-2 Gene Expression in Inhibiting The Growth of Hepatocellular Carcinoma of Nude Mice by Quercetin

作　　者：张继红[1] 梁力建[2] 黄洁夫[2] 

机构地区：[1]中山大学附属第一医院黄埔院区肝胆外科,广州510070 [2]中山大学附属第一医院肝胆外科,广州510080

出　　处：《中国普外基础与临床杂志》2009年第6期455-459,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：广东省医学科学基金立项课题(项目编号:A2004537)~~

摘　　要：目的探讨三磷酸肌醇(IP3)和bcl-2基因表达变化在槲皮素治疗裸鼠移植人肝细胞癌中的作用。方法建立裸鼠移植人肝细胞癌模型,对照组腹腔注入含0.4% DMSO的RPMI 1640培养基0.05ml/(g·d),槲皮素治疗组腹腔注入槲皮素50mg/(kg·d),3周后观察肝癌生长情况,并应用IP3-[3H]Birtrak Assay试剂盒检测肝癌组织中IP3的含量,RT-PCR检测肝癌组织中bcl-2 mRNA的表达,Western blot检测肝癌组织中bcl-2蛋白的表达。结果槲皮素治疗组移植瘤体积和重量均明显小于或轻于对照组体积:(15.8±10.1)mm3比(52.3±26.5)mm3,重量:(44.8±10.4)mg比(91.3±31.4)mg,P<0.01;肝癌组织中IP3含量明显低于对照组(13.4±1.4)pmol/mg prot比(35.3±6.6)pmol/mg prot,P<0.01,bcl-2 mRNA表达低于对照组但差异无统计学意义RI(相对灰度与面积之积):0.55±0.05比0.79±0.19,P>0.05,bcl-2蛋白的表达则明显低于对照组RI:1.07±0.12比6.69±1.80,P<0.01。结论槲皮素能减少IP3的生成,下调肝癌组织中bcl-2基因的表达,抑制裸鼠移植人肝细胞癌的生长。Objective To probe into the roles of inositol 1, 4, 5-trisphosphate （IP3） and bcl-2 gene expression in inhabiting hepatocellular carcinoma of nude mice by quercetin. Methods Animals with hepatocellular carcinoma in quercetin group were treated with injection peritoneum of quercetin 50 mg/（kg · d ） for 3 weeks, while which in control group were treated with 0.4% DMSO of RPMI 1640 0.05 ml/（g · d）. Then the volume and the weight of tumors were measured, IP3 , bcl-2 mRNA and bel-2 protein were assayed by IP3-[^3 H] Birtrak Assay, RT- PCR and Western blot respectively. Results The volume and weight of tumors in quercetin group were lower than those in control group [（15.8±10.1） mm^3 vs. （52.3±26.5） mm^3 in volume, （44.8±10.4） mg vs. （91.3±31.4） mg in weight, P〈0. 01]. Content of IP3 in quercetin group was lower than that in control group [（13.4 ±1.4） pmol/mg prot vs. （35.3±6.6） pmol/mg prot, P〈0.01]. There was no significant difference in bcl-2 mRNA expression between quercetin group and control group [RI （the gray degree multiply area of bcl-2 /the gray degree multiply area of β-actin） ： 0.55±0.05 vs. 0. 79±0.19, P〉0.05], but the expression of bcl-2 protein in quercetin group was lower than that in control group （RI： 1.07±0. 12 vs. 6.69± 1.80, P〈0.01）. Conclusion Quercetin can inhabit the growth of hepatocellular carcinoma tansplanted into liver of nude mice by reducing IP3 production and down-regulating bcl-2 gene expression.

关 键 词：肝细胞癌 槲皮素 BCL-2基因 三磷酸肌醇 信号转导 

分 类 号：R735.7[医药卫生—肿瘤]