单克隆与多克隆双抗体夹心法测定可溶性白细胞介素2受体试剂盒的研制  

Development of ELISA Kit Using Monoclonal and Polyclonal Antibody to Determine the Level of Soluble Interleukin-2 Receptor

作　　者：邬义安[1] 王志友[1] 蒋轶敏[1] 端义坤[1] 李基础 史良如[1] 

机构地区：[1]卫生部武汉生物制品研究所免疫研究室,武汉430060

出　　处：《同济医科大学学报》1998年第3期187-189,共3页Acta Universitatis Medicinae Tongji

摘　　要：采用抗白细胞介素2受体(IL-2R)的单克隆与多克隆抗体以及辣根过氧化物酶(HRP)标记的羊抗兔IgG,建立了高特异性及高灵敏度的双抗体夹心法测定可溶性白细胞介素2受体(sIL-2R)。结果,酶联免疫吸附试验(ELISA)测得兔抗人IL-2R多克隆抗体效价达1:10~4,免疫双向扩散法测得羊抗兔抗体效价高达1:256。本试剂盒检测sIL-2R的特异性强,灵敏度为100U/ml,在28C存放一年,稳定性不变。此方法操作简单,适用于临床和基础研究。Monoclonal antibody (McAb) and polyclonal antibody to interleukin-2 receptor (IL-2R) and the second antibody labeled with horse radish peroxidase (HRP) were used to develop a high specific and sen-sitive sandwich type of enzyme-linked immunosorbent assay (ELISA) method to determine soluble IL-2R (sIL-2R). The results indicated that the titre of polyclonal antibody for rabbit to human IL-2R was 1: 10~4 by using ELISA, and the titre of the second antibody for goat to rabbit was 1 : 256 by means of immune diffu-sion test. The developed kit a high specificity for the determination of sIL-2R with the sensitivity being 100 U/ml. The kit remained stable stored in 2 C^8 C for one year. This assay method is simple and can be ap-plied to clinical practice and basic research.

关 键 词：单克隆抗体 多克隆抗体 双抗体夹心法 SIL-2R 

分 类 号：R392.11[医药卫生—免疫学]