齐墩果酸预处理对大鼠肝脏缺血再灌注时糖原含量的影响  

Effects of pretreatment with oleanolic acid on glycogen content in rats during hepatic ischemic reperfusion

作　　者：许仄平[1] 张杰[1] 钱燕宁[2] 孙宏斌[2] 陆森[3] 

机构地区：[1]南京医科大学第一附属医院麻醉科,江苏南京210029 [2]中国药科大学新药研究中心,江苏南京210009 [3]南京医科大学第一附属医院肝脏外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2009年第7期930-933,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30672523)

摘　　要：目的:探讨齐墩果酸(OA)预处理对大鼠肝脏缺血再灌注(IR)时糖原含量的影响及其意义。方法:128只雄性SD大鼠随机均分为假手术组(A组),IR组(B组),0.5%羧甲基纤维素钠组(CMC-Na)(C组),0.5%CMC-Na和OA的混悬液组(D组)。术前D组以100mg/kg的OA混悬液,A、B组以相同容积水,C组以相同容积的单纯0.5%CMC-Na分别每日灌胃1次,连续7日。第8日建立70%肝脏IR(缺血60min后行再灌注)模型,分别测定术前,再灌注0、3、6h血清丙氨酸转移酶(ALT)活性、肝组织的糖原含量和乳酸值,并在光镜下观察肝组织病理学改变。结果:术前D组糖原含量分别高于其他各组,差异有显著性(P<0.05),再灌注0、3、6h,B、C、D组糖原含量分别低于A组,差异有显著性(P<0.05),但D组糖原含量分别高于B和C组,差异有显著性(P<0.05)。再灌注0hD组乳酸值分别高于其他各组,差异有显著性(P<0.05);B和C组乳酸值分别高于A组,差异有显著性(P<0.05)。再灌注3、6h,B、C、D组ALT活性均高于A组,差异有显著性(P<0.05);D组ALT活性分别低于B和C组,差异有显著性(P<0.05);D组肝脏病理形态学改变分别较B、C组减轻。结论:OA预处理可增加大鼠肝脏的糖原含量,减轻肝脏IR时的损伤,其机制可能与增加IR时的能量供给有关。Objective：To investigate the effect of pretreatment with oleanolic acid （OA） on glycogen content during hepatic ischemic reperfusion（IR）in rats. Methods ：128 Male Sprague-Dawley （SD）rats were randomly devided into sham（A）,IR（B）,0.5% sodium carboxymethycellulose（CMC-Na）（C）,OA＋0.5% CMC-Na（D）equally. Before operation,all rats got intragastric administration once a day for 7 days, group D with OA （100 mg/kg）＋0.5% CMC-Na,group A,B with water,group C with 0.5% CMC-Na in same volume. At the 8th day,rats suffered from segmental （70%）hepatic ischemia for 60 min and were followed by different periods of reperfusion. Blood and liver tissues were collected and measured respectively at preoperation, 0,3 and 6 h after reperfusion for ALT, glycongen and lactate. And changes of histological pathology were also observed by light microscope. Results：Before operation, glycongen content of group D increased compared with other groups （P〈 0.05）. After reperfusion glycogen content in grup A decreased at 0,3,6 h compared with group B,C and D （P 〈 0.05）;glycongen content in group D increased compared with group B and C respectively （P 〈 0.05）. At 0 h after reperfusion,lactate level of group D increased compared with other groups （P 〈 0.05） ;lactate level of group B and C increased compared with group A （P 〈 0.05）. At 3,6 h after reperfusion,the activity of ALT of group B,C and D increased compared with group A（P 〈 0.05）;the activity of ALT in group D decreased compared with group B and C respectively（P 〈 0.05） ;group D had relieved morphologic changes compared with group B and C. Conclusion：OA pretreatment could increase glycogen content,also provide the rat liver with significant tolerance to IR injury. The mechanism may be related to the increased level of energy during IR.

关 键 词：齐墩果酸 肝脏 缺血再灌注 糖原 

分 类 号：R362[医药卫生—病理学]