肝细胞肝癌中survivin、caspase-3和NF-κB的表达及其相关性  

Expressions of survivin,caspase-3 and NF-κB in hepatocellular carcinoma and their correlation

作　　者：秦安[1] 蔡兵[1] 王彤[1] 杨国仪[2] 夏钰弘[2] 梁加贝[2] 

机构地区：[1]南京医科大学附属无锡市人民医院普外科,江苏无锡214023 [2]南京医科大学附属无锡市人民医院病理科,江苏无锡214023

出　　处：《南京医科大学学报（自然科学版）》2009年第7期953-957,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅指导性科研课题(Z200718);南京医科大学科技发展基金项目(07NMUZ032)

摘　　要：目的:探讨survivin、caspase-3、NF-κB在肝细胞肝癌(HCC)中的表达,及其与临床病理指标的关系。方法:采用免疫组织化学技术检测40例HCC组织及10例正常肝组织中survivin、caspase-3、NF-κB的表达情况。结果:survivin在HCC组织阳性表达率为67.5%,在对照组中不表达,两者比较有显著性差异(χ2=12.082,P<0.05);门静脉癌栓者阳性表达率高于无门静脉癌栓者(χ2=5.405,P<0.05);有远处转移者阳性表达率高于无远处转移者(χ2=4.596,P<0.05)。caspase-3在HCC组织阳性表达率为35.0%,在对照组中表达率为80.0%,两者比较有显著性差异(χ2=4.875,P<0.05)。NF-κB在HCC组织阳性表达率为77.5%,在对照组中不表达,两者比较有显著性差异(χ2=17.238,P<0.05);中低分化阳性表达率高于高分化(χ2=4.198,P<0.05)。caspase-3的表达与survivin的表达呈负相关(r=-0.679,P<0.05);NF-κB的表达与survivin的表达呈正相关(r=0.519,P<0.05)。结论:survivin、caspase-3、NF-κB在HCC的发生、发展中起着不同程度的作用;联合检测survivin、caspase-3、NF-κB,有助于对HCC恶性程度的判定及侵袭转移能力的评估,进而为HCC的预后分析及临床治疗提供依据。Objective：To investigate the expressions of survivin,caspase-3 and NF-kB in hepatocellular carcinoma （HCC）and their relationship with clinical pathologic features of HCC. Methods：40 cases of HCC specimens were collected as study group and 10 cases of normal specimens as control. Immunohistoehemeal method was used to detect the expressions of survivin,caspase-3 and NF-kB. Results：Positive expression rate of survivin was 67.5% in HCC study group,while negative in the control. There was significant difference between the study and normal control groups （X^2=12.082,P 〈 0.05）. In the study group,the positive expression rate of survivin was higher in the group with oncogenic portal vein thrombosis than in the group without （X^2=5.405,P 〈 0.05）. The positive rate of survivin was higher in the cases with extrahepatic metastasis than in eoses without （X^2=4.596, P 〈 0.05）. 35.0% of the study group expressed caspase-3 ,while 80.0% of the control group expressed easpase-3. Significant difference was found between these two groups （X^2=4.875,P 〈 0.05）. 77.5% of the experimental group expressed NF-kB while negative in the control. There was significant difference between these two groups （X^2=17.238,P 〈 0.05）. The positive expression rate of NF-kB was higher in the low differentiation group than those in the high differentiation group （x^2=4.198, P 〈 0.05 ）. There was negative correlation between the expression of caspase-3 and survivin（r=-0.679 ,P 〈 0.05）. However, the correlation between the expression of NF-kB and survivin was positive （r=0.519,P 〈 0.05）. Conclusion：Since survivin,caspase-3 and NF-kB each has a different effect on the origin and development of HCCs, the combination of detection of these three factors will provide us a valuable clinical index for the degree of malignancy and capability of metastasis,it offers HCC a better treatment regimen and prognosis.

关 键 词：肝细胞肝癌 SURVIVIN CASPASE-3 NF-KB 免疫组织化学 

分 类 号：Q786[生物学—分子生物学]