酶底物法快检技术在四川5.12地震灾区生活饮用水监测中的应用  

Rapid detection method of enzyme substrate technique in drinking water in Sichuan 5.12 earthquake-stricken area

作　　者：蔡炯[1] 周蕾[1] 梁娜[1] 黄薇[1] 孟建彤[1] 胡小为[1] 许成华[1] 李佳[1] 白艳[1] 唐晓旻[1] 李涛[1] 黎明[1] 苗艳芳[1] 时炜[1] 

机构地区：[1]成都市疾病预防控制中心,成都610041

出　　处：《中国卫生检验杂志》2009年第6期1327-1328,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:在四川5.12抗震救灾时期,开展了灾区生活饮用水的卫生监测工作,由于时间和人力资源的限制,对微生物快速检测提出了要求。方法:运用酶底物法快速检测生活饮用水中三项微生物指标:大肠菌群、耐热大肠菌群、大肠埃希菌。结果:共检测灾区生活饮用水2000余件,其中市政供水350余件,合格率约94%;乡镇供水1650余件,合格率约90%。酶底物法24 h可报告结果,并能同时检测2个指标,实现了快速检测的目的。结论:酶底物法作为快速检测技术,在此次生活饮用水监测工作中发挥了突出的作用,极大地节约了时间和人力,有力地支持了抗震救灾工作的顺利实施,保障了广大灾区人民群众生活饮用水的供水安全。Objective： Health monitoring in drinking water in Sichuan 5. 12 earthquake - stricken area had been carried out. The rapid detection methods in micro -organisms were needed for time and labor limitations. Methods：Use enzyme substrate technique to detect total coliforms, thermotolerant coliform bacteria and Escherichia coli in drinking water. Results： There were more than 2000 samples have been detected. And in which, 350 samples from municipal water supply with a pass rate of about 94%, and 1650 samples from township water supply with a pass rate of about 90%. Enzyme substrate technique is a rapid detection method for it can make results within 24 hours and can detect two items at the same time. Conclusion：The enzyme substrate technique can obviously save time and labor. It has been successfully applied in detecting drinking water in Sichuan 5. 12 earthquake - stricken area.

关 键 词：酶底物法 大肠菌群 生活饮用水 快速检测 

分 类 号：R446.5[医药卫生—诊断学]