丙型肝炎病毒C基因在E.coli中的高效表达  

High level expression of HCV nucleocapsid gene in E.coli

作　　者：逯好英[1,2,3] 金灵[1,2,3] 王宇 王捷[1,2,3] 张晓晖[1,2,3] 尚丹 马贤凯[1,2,3] 

机构地区：[1]军事医学科学院基础医学研究所 [2]北京医科大学肝病研究所 [3]广州军区总医院医学实验科

出　　处：《军事医学科学院院刊》1998年第2期94-97,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家"863"高科技资助

摘　　要：目的：通过观察影响ＨＣＶＣ基因在大肠杆菌中表达的因素，研制高表达工程菌株，制备重组抗原，用于免疫诊断和生物学功能研究。方法：将２种不同大小的ＨＣＶＣ基因片段克隆到带有ＰＬ启动子的表达载体中，通过ＳＤＳ－ＰＡＧＥ和免疫印迹分析基因表达产物。结果：在观察影响表达因素的基础上，获得了高表达工程菌株，其基因表达产物占菌体蛋白的３８．５％。结论：本研究表明。Objective: In order to obtain overexpression product for development of immunodiagnostic assay and study its biological functions, some factors affecting the expression of HCV nucleocapsid gene in E.coli system were observed. Methods: Two gene fragments of the HCV nucleocapsid differing in length were cloned into expression vector with PL promoter. The expressed products were analyzed by SDS PAGE and Western immunoblotting. Results: High level expression was achieved in E.coli system. The expressed product of the gene fragment lacking the hydrophobic carboxyterminus of the HCV nucleocapsid could amount to 38.5% of total bacterial protein in optimum host cells. Conclusion: This study suggests that the gene fragment lacking the hydrophobic carboxyterminus of the HCV nucleocapsid be expressed efficiently in E.coli system.

关 键 词：丙型肝炎病毒 C基因 基因表达 疏水区编码序列 

分 类 号：R512.63[医药卫生—内科学] Q78[医药卫生—临床医学]