原发性胆汁性肝硬化患者血清中Th17相关细胞因子的表达  

Serum profile of Th17-related cytokines in patients with primary biliary cirrhosis

作　　者：周运恒[1] 荣光华[1] 熊怡淞[1] 朱烨[1] 耿红莲[1] 杨再兴[1] 仲人前[1] 

机构地区：[1]第二军医大学长征医院实验诊断科,全军医学免疫诊断中心,全军临床免疫重点实验室,上海200003

出　　处：《第二军医大学学报》2009年第6期703-705,共3页Academic Journal of Second Military Medical University

基　　金：国家高科技研究发展计划("863"计划;2006AA02Z496);上海市科学技术委员会优秀学科带头人基金(07XD14013)~~

摘　　要：目的:观察原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者血清中Th17分化相关细胞因子(IL-1β、IL-6、IL-17、IL-23、TGF-β)的变化,探讨Th17细胞在PBC发病过程中的作用。方法:采用酶联免疫吸附试验(ELISA)方法比较PBC患者(n=78)、乙型肝炎后肝硬化患者(PHC,n=30),健康对照(HC,n=60)血清中Th17分化相关细胞因子含量。结果:与HC组相比,PBC患者血清IL-1β、IL-6、IL-17A和IL-23水平显著升高(P<0.05),而TGF-β水平差异无统计学意义;与PHC组相比,PBC患者血清IL-1β、IL-6和TGF-β水平显著降低,而IL-17和IL-23水平显著升高(P<0.05)。结论:PBC患者处于慢性炎症状态,Th17细胞可能在PBC的发病和发展过程中发挥着重要的作用。Objective：To observe the serum profile of Th17 related cytokines（IL-1β, IL-6, IL-17A, IL-23, and TGF-β）in patients with primary biliary cirrhosis, so as to study the role of Th17 cells in the pathogenesis of primary biliary cirrhosis （PBC）. Methods：Seventy-eight patients with PBC, 30 patients with HBV-related posthepatitic cirrhosis（PHC）, and 60 healthy controls （HC） were enrolled in this study. The serum levels of above five Th17-related cytokines were determined by enzymelinked immunosorbent assay （ELISA）. Results： The levels of IL-1β, IL-6, IL-17A, and IL-23 in PBC group were significantly higher than those in HC group （P〈0.05） ; the levels of TGF-β were comparable between the PBC group and HC group. The levels of IL-1β, IL- 6, and TGF-β were significantly lower and IL-17A, IL-23 were significantly higher in the PBC group compared with those in the PHC group （P〈0.05）. Conclusion：Our findings indicate that PBC patients are in a status of chronic inflammation. Th17 subset may play an important role in the development and progression of PBC.

关 键 词：原发性胆汁性肝硬化 TH17细胞 细胞因子 

分 类 号：R575.22[医药卫生—消化系统]