促红细胞生成素对胰腺癌细胞株SW1990吉西他滨化疗敏感性的影响  

Effect of erythropoietin on chemo-sensitivity of pancreatic cancer cell line SW1990 to gemcitabine

作　　者：杨琼[1] 谢德荣[1] 江志敏[1] 陈邓林[1] 毕卓菲[1] 马雯[1] 

机构地区：[1]中山大学附属第二医院肿瘤化疗专科,广州510120

出　　处：《中华胰腺病杂志》2009年第3期178-180,共3页Chinese Journal of Pancreatology

基　　金：广东省科技计划项目（2007B031516014）

摘　　要：目的观察促红细胞生成素（Epo）对胰腺癌细胞株SW1990增殖活性和吉西他滨化疗敏感性的影响，并初步探讨其可能的机制。方法免疫细胞化学法检测SW1990中促红细胞生成素受体（EpoR）的表达；采用不同浓度Epo干预SW1990细胞，MTT法检测细胞增殖及对吉西他滨化疗敏感性的变化，RT-PCR法检测多药耐药基因（MDR-1）、核苷酸还原酶亚单位M1（RRM1）的表达。结果EpoR在胰腺癌SW1990细胞中表达。0、2、4、10U／ml Epo干预组的A630值分别是0．597±0．043、0．645±0．073、0．690±0．094、0．630±0．073，差别无统计学意义；吉西他滨对SW1990的抑制率分别是（64．7±0．142）％、（35．6±0．076）％、（33．1±0．140）％、（29．9±0．082）％；SW1990细胞MDR-1mRNA相对表达量分别为0．42±0．10、0．58±0．08、0．60±0．04、0．68±0．13；RRM1 mRNA相对表达量分别为1．32±0．17、1．64±0．15、1．57±0．11、1．67±0．18。2、4、10U／ml Epo处理组对SW1990生长抑制率较0浓度组显著降低，而MDR-1 mRNA、RRM1 mRNA表达量增加（P〈0．01或〈0．05）。吉西他滨对SW1990的抑制率与MDR-1 mRNA和RRM1 mRNA表达均呈负相关（r=-0．964，P=0．036；r=-0．968，P：0．032）。结论Epo可降低胰腺癌SW1990细胞对吉西他滨的化疗敏感性，其机制可能是上调MDR-1、RRM1的基因表达水平。Objectives To investigate the effect of erythropoietin （Epo） on cellular proliferation and chemo-sensitivity to gemcitabine of pancreatic cancer cell line SW1990, and to explore the possible mechanisms. Methods The expression of EpoR in SW1990 was detected by immunoeytochemical method, cancer cell line SW1990 was interfered by different concentrations of Epo, the cellular proliferation and ehemosensitivity to gemcitabine of SW1990 was detected by MTT method, the expression of MDR-1 mRNA, RRM1 mRNA was detected by RT-PCR. Results EpoR was expressed in SW1990. The average A630 were 0. 597 ± 0.043, 0. 645 ± 0. 073, 0.690 ± 0. 094, 0.630 ± 0. 073 in 0, 2, 4, 10 U/ml Epo groups, respectively, but the difference didn＇t reach statistical significance. The average inhibitory rate （IR） in Epo treatment groups were （64.7 ±0. 142）%, （35.6 ±0.076）%, （33. 1 ±0. 140）%, （29.9 ±0.082）%, MDR-1 mRNA expressions were 0.42 ± 0.10, 0.58 ± 0.08, 0.60 ± 0.04, 0.68 ± 0.13 ; RRM1 mRNA expressions were 1.32 ±0.17, 1.64±0.15, 1.57±0.11, 1.67±0.18. The IRs of SW1990 in 2, 4, 10 U/ml Epogroups were statistically lower than 0 U/ml Epo group, while the expressions of MDR-1 mRNA, RRM1 mRNA were significantly increased （P 〈 0.01 or 〈 0.05）. The IR of gemcitabine on SW1990 was negatively related with the expressions of MDR-1 mRNA, RRM1 mRNA （r = -0. 964, P =0.036; r = -0.968, P=0.032）. Conclusions Epo might decrease the ehemo-sensitivity of SW1990 to gemeitabine possibly by up-regulating the expressions of MDR-1 and RRM1.

关 键 词：胰腺肿瘤 红细胞生成素 吉西他滨 基因 MDR 核苷酸还原酶亚单位M1 

分 类 号：R735.9[医药卫生—肿瘤]