吡咯烷二硫氨基甲酸酯对大鼠急性坏死性胰腺炎的影响  

Effect of pyrrolidinecarbodithioic on rats with severe acute pancreatitis

作　　者：张东兵 王益林 张紫平 

机构地区：[1]闵行区中心医院普外科,上海201100

出　　处：《中华胰腺病杂志》2009年第3期198-200,共3页Chinese Journal of Pancreatology

摘　　要：目的探讨抗氧化剂吡咯烷二硫氨基甲酸酯（PDTC）对大鼠急性坏死性胰腺炎（ANP）的影响及其可能的机制。方法将36只Wistst大鼠按数字表法分为假手术组、假手术＋静脉注射组、ANP组和ANP＋静脉注射组。ANP组经胆胰管逆行注入3％的牛磺胆酸钠0．1ml/100g体重制模；假手术组仅开腹轻度揉搓胰腺；静脉注射为术前30min经尾静脉注射PDTC25mg／kg体重。术后6h处死大鼠，取血测定血清淀粉酶及脂肪酶含量，取胰腺组织进行病理学评分，采用免疫组化法检测NF—κB活化水平及胰腺细胞凋亡指数。结果假手术组、假手术＋静脉注射组、ANP组、ANP＋静脉注射组血清淀粉酶含量分别为（3121±34）U／L、（2985±89）U／L、（7422±99）U／L和（5330±94）U／L；脂肪酶含量为（34±11）U／L、（36±11）U／L、（566±167）U／L和（363±132）U／L；胰腺病理分值为0、0、22．13±0．61和17．38±0．53；NF—κB阳性率为0、0、（66．7±4．2）％和（38．6±2．7）％；凋亡指数为0、0、（7．80±0．56）％和（13．30±0．73）％。ANP组各项指标均显著高于假手术组（P〈0．05）；单纯PDTC注射对各项指标没有影响，但PDTC干预ANP后，各项指标与ANP组均有显著差异（P〈0．05）。结论PDTC可以减轻胰腺组织的病理损害，其机制可能是抑制胰腺细胞NF—κB的激活，促进胰腺细胞凋亡，减少胰酶释放。Objective To investigate the effect of pyrro-2-lidinecarbodithioic （PDTC） on rat with acute necrotizing pancreatitis （ANP）. Methods Thirty-six Wistar rats were randomly divided into 4 groups： sham operation group （ n = 6）, sham operation plus intravenous injection group （ n = 6）, ANP group （ n = 12） and ANP plus intravenous injection group （n = 12）. ANP were induced by retrograde injection of 3% sodium taurocholate （0.1 ml/100g weight） into the biliopancreatic duct. Rats of sham operation group only underwent opening the abdomen and rubbing the pancreas. Rats of intravenous injection group received intravenously 25 mg/kg body weight PDTC. After 6h of establishment of the ANP model, the rats were sacrificed and the serum levels of amylase and lipase were determined. Pancreatic tissues were examined for pathological score. The activation levels of NF-κB and apoptosis index of pancreatic cells were detected by immunohistochemistry. Results The serum levels of amylase in sham operation group, sham operation plus intravenous injection group, ANP group and ANP plus intravenous injection group were （3 121 ±34）U/L, （2 985 ±89）U/L, （7 422 ± 99 ） U/L and （ 5 330 ±94 ） U/L, respectively ; while the serum levels of lipase were （ 34 ± 11 ） U/L, （ 36 ± 11 ） U/L, （566 ± 167 ） U/L and （ 363 ± 132 ） U/L, respectively; pathological scores were 0, 0, 22.13 ± 0.61 and 17.38 ±0.53, respectively; the positive rates of NF-κB were 0, 0, （66.7 ±4.2）% and （38.6 ±2.7）%, respectively; apoptosis index were 0, 0, （7.80 ±0.56） % and （ 13.30 ±0.73） %, respectively; all the values in the ANP group were significantly higher than those in sham operation group （ P 〈 0.05 ）, PDTC injection alone had no effect on all the values, however, after PDTC injection, all the values were significantly lower than those in ANP group （P 〈 0.05 ）. Conclusions PDTC could alleviate pancreatic tissue damage possibly by inhibiting the activation of NF-κB, accele

关 键 词：胰腺炎 急性坏死性 核因子ΚB 脱噬作用 吡咯烷二硫氨基甲酸酯 

分 类 号：R576[医药卫生—消化系统]