磁感应热疗用镍-铜热籽对L-929细胞及兔肌肉组织的影响  

Effect of Ni-Cu Thermoseeds on L-929 Cells and Muscle Tissues in Rabbits

作　　者：谢小雪[1] 高福平[2] 白雪[3] 唐劲天[2] 

机构地区：[1]中南大学湘雅医学院肿瘤科,410008 [2]清华大学工程物理系医学物理与工程研究所,100084 [3]佳木斯大学口腔医学院,154007

出　　处：《中国微创外科杂志》2009年第6期481-486,共6页Chinese Journal of Minimally Invasive Surgery

基　　金：国家自然科学基金(10775085);国家自然科学基金(30571779);北京市科委(Z07000200540704);清华大学裕元基金

摘　　要：目的探讨磁感应热疗用镍-铜热籽体外升温特性以及对细胞与组织的影响。方法测量感应热籽在离体兔肝组织内升温特性;通过细胞毒性试验(MTT试验)评价该治疗热籽浸提液体外细胞毒性;溶血试验评价其有无溶血作用;兔体内肌肉埋植试验来评价其材料的组织毒性。结果实验中所用的铁磁热籽在离体兔肝内具有良好的升温效果;细胞毒性试验结果显示镀金热籽对L-929细胞毒性为1级,属对细胞无毒性范畴;溶血试验中镀金热籽的溶血率3.25%(<5%),表明实验用热籽无溶血作用;各期体内埋植试验反映了材料在肌肉组织中不同的炎性反应。结论自制的镀金镍-铜热籽在感应加温交变磁场中可升温到适合的温度,对L-929细胞毒性为1级,无溶血作用。Objective To study the in vitro heating ability of Ni-Cu thermoseeds and their effect on the rabbit liver cells and tissues. Methods The temperature of rabbit liver tissues were monitored under an alternating magnetic field. MTT assay was used to evaluate the in vitro eytotoxieity of the extra-liquid of the Ni-Cu thermoseeds; Hemolytic test was carried out to estimate its blood toxicity ; and muscular implantation test was employed to determine the levels of its tissue toxicity. Results The thermoseeds used in this experiment showed a high heating ability in alternating magnetic field in vitro. MTT assay showed that the toxicity of the material on mouse fibroblast （L-929） cell lines was 1 degree, which means non-eytotoxic. Hemolytic test revealed a hemolysis rate （HR） of 3.25% （less than 5% ） , showing that the thermoseeds had no hemolytic reaction. Muscular implantation test showed different levels of inflammatory reaction in the muscle tissues. Conclusion Thermoseeds induced heating in alternating magnetic field can achieve an appropriate temperature, and the gilded thermoseeds have a high biocompatibility with 1 degree cytotoxicity without leading to hemolytic reaction.

关 键 词：感应加温 热籽 升温效应 生物相容性 L-929细胞 

分 类 号：R730.5[医药卫生—肿瘤]