钙结合蛋白S100A8/A9对宫颈癌细胞系CasKi细胞F-肌动蛋白的影响  

Effect of S100A8/A9 Protein Complex on F-actin Network in Human Cervical Carcinoma Cell Line,CasKi Cells

作　　者：王希涛[1] 孙全梅[2] 张幼怡[3] 陈嵘[1] 韩东[2] 耿力[1] 

机构地区：[1]北京大学第三医院妇产科,北京100191 [2]国家纳米科学中心纳米生物医学成像与表征实验室,北京100190 [3]北京大学第三医院血管医学研究所,北京100191

出　　处：《中国微创外科杂志》2009年第6期556-559,共4页Chinese Journal of Minimally Invasive Surgery

基　　金：国家自然科学基金资助(No:10772007和30571939);国家重点基础研究"973"发展规划项目资助肿瘤细胞和心肌细胞的纳米力学特征与表征方法(2007CB935602)

摘　　要：目的探讨钙结合蛋白S100A8/A9复合物对人宫颈癌上皮细胞癌细胞系CasKi细胞骨架及表面超微结构的影响。方法应用20μg/ml S100A8/A9复合物作用于CasKi细胞,对细胞骨架F-肌动蛋白进行染色,应用快速共聚焦荧光显微镜观察细胞骨架的改变。在生理条件下,应用原子力显微镜(atomic force microscopy,AFM)对活体细胞进行高分辨成像,观察细胞表面超微结构及应力纤维。结果S100A8/A9复合物作用24 h后宫颈癌细胞系CasKi细胞的F-肌动蛋白排列出现紊乱,主要分布于细胞的周边,细胞中央F-肌动蛋白含量明显减少。CasKi细胞经过20μg/ml S100A8/A9蛋白复合物处理前细胞核部位F-肌动蛋白光密度为92.42±5.16,经过S100A8/A9 72 h处理后,F-肌动蛋白的光密度降为57.67±3.70,两者相比较差异具有显著的统计学意义(t=5.268,P=0.000)。AFM成像显示S100A8/A9复合物作用后细胞边缘卷曲,高度增加,伪足减少,细胞膜下应力纤维结构遭到破坏。结论S100A8/A9蛋白复合物可以对宫颈癌细胞系CasKi细胞表面的超微结构及应力纤维产生影响,并且这种影响主要是通过肌动蛋白的重新分布实现的。Objective To investigate the effect of SI00A8/A9 protein complex on the surface morphology and the F-actin network in human cervical carcinoma cell line, CasKi cells. Methods After being cultured with 20 μg/ml S100A8/A9 protein complex, the cell skeleton of the CasKi cells were observed under a confoeal scanning fluorescence microscope by staining the F-aetin network. Atomic force microscopy （AFM） was employed to reveal the change of ultrastrueture of the cell surface in vivo. Results After being cultured with the S100A8/A9 protein complex for 24 hours, the F-aetin network disorder was revealed. Most of the F-actins distributed peripherally. The OD value of the F-aetin decreased significantly from 92.42 ± 5.16 to 57.67 ± 3.70 after been treated with the S100A8/A9 （t = 5. 268, P = 0. 000）. The AFM showed a withdrawing morphology with reduced pseudopodia and destruction of stress fibers. Conclusion S100A8/A9 protein complex can change the ultrastructure of the surface of CasKi cells and its stress fibers by re-distributing of the F-actin in the cells.

关 键 词：原子力显微镜 钙结合蛋白S100A8/A9 CASKI细胞 宫颈癌 F-肌动蛋白 

分 类 号：R737.33[医药卫生—肿瘤]