低密度脂蛋白对大鼠肝贮脂细胞的调控作用  

Modulating the hepatic fat-storing cells activity by low density lipoprotein in rats

作　　者：李定国[1] 王炯[1] 陆汉明[1] 孙志广[1] 徐芹芳[1] 蒋祖明[1] 顾鹤定[1] 李兆东[1] 

机构地区：[1]上海第二医科大学新华医院

出　　处：《中华消化杂志》1998年第3期148-149,共2页Chinese Journal of Digestion

摘　　要：目的研究低密底脂蛋白（ＬＤＬ）对大鼠肝贮脂细胞（ＦＳＣ）的调控作用。方法采用放射配体结合法检测肝ＦＳＣ是否存在ＬＤＬ受体。通过噻唑蓝（ＭＴＴ）法、透明质酸（ＨＡ）放射免疫分析法及３Ｈ－脯氨酸掺入法测定ＬＤＬ对ＦＳＣ增殖、ＨＡ及胶原合成的影响。结果大鼠肝脏ＦＳＣ上存在特异性的、高度亲和性的及可饱和性的ＬＤＬ结合位点：ＬＤＬ能促进ＦＳＣ增殖、ＨＡ及胶原合成。结论ＬＤＬ参与ＦＳＣ增殖、转化和基质合成，并可能通过受体介导影响ＦＳＣ功能。Objective To study the effects of low density lipoprotein (LDL) on modulation of hepatic fat-storing cells (FSC) activity in rats . Methods Radio- ligand-binding assay were used to determine the existence of LDL receptor-binding sites on FSC surface. MTT colorimetric assay, hyaluronic acid radioimmunoassay and the technique of incorporation of 3H-proline were used to observe the effects of LDL on the proliferation, HA and collagen synthesis of FSC . Results There exist the LDL receptor-binding sites on FSC surface which were specific, highly affinitive and saturable. LDL could enhance the proliferation of FSC, as well as their synthesis of HA and collagen. Conclusion LDL are involved in the proliferation, transforma- tion and the synthesis of extracellular matrix of FSC, these can modulate the activity of FSC by their receptors.

关 键 词：肝纤维化 贮脂细胞 低密度脂蛋白 透明质酸 

分 类 号：R575.202[医药卫生—消化系统]